A novel variant of translation elongation factor-1β:: isolation and characterization of the rice gene encoding EF-1β2

被引：7

作者：

Terui, Y

Tsutsumi, K

Kidou, S ^{[1
]}

Sawazaki, T

Kuroiwa, Y

Yamaki, M

Ejiri, S

机构：

[1] Iwate Univ, Fac Agr, Cryobiosyst Res Ctr, Morioka, Iwate 0208550, Japan

[2] Hitachi Chem Co Ltd, Pharmaceut Res Lab, Hitachi, Ibaraki 317, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1442卷 / 2-3期

关键词：

elongation factor-1 beta; rice; gene; cDNA; expression; translation;

D O I：

10.1016/S0167-4781(98)00153-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rice gene encoding a novel isoform of translation elongation factor-1 beta subunit (termed EF-1 beta 2) was isolated and characterized. The gene comprises of eight exons, and encodes a 226-amino-acid protein. Expression of EF-1 beta 2 mRNA is abundant in seeds and cultured cells, but is considerably low in the tissues of the rice seedling. Antiserum raised against an EF-1 beta 2 synthetic peptide detected a protein with a relative molecular mass of about 32 kDa, indicating the EF-1 beta 2 gene is actually expressed in rice tissues. EF-1 beta 2 showed a close similarity to the cognate subunits from plant (beta and beta'). (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：369 / 372

页数：4

共 16 条

[1] THE LEUCINE-ZIPPER IN ELONGATION-FACTOR EF-1-DELTA, A GUANINE-NUCLEOTIDE EXCHANGE PROTEIN, IS CONSERVED IN ARTEMIA AND XENOPUS [J].