Neural-specific elongation of 3′ UTRs during Drosophila development

被引：120

作者：

Hilgers, Valerie ^{[1
]}

Perry, Michael W. ^{[1
]}

Hendrix, David ^{[1
]}

Stark, Alexander ^{[2
]}

Levine, Michael ^{[1
]}

Haley, Benjamin ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Ctr Integrat Genom, Div Genet Genom & Dev, Berkeley, CA 94720 USA

[2] Res Inst Mol Pathol, A-1030 Vienna, Austria

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 38期

基金：

奥地利科学基金会; 美国国家卫生研究院;

关键词：

posttranscriptional process; maternal-to-zygotic transition; nervous system; alternative polyadenylation; HUNCHBACK MESSENGER-RNA; EMBRYONIC-DEVELOPMENT; UNTRANSLATED REGIONS; FUNCTIONAL ELEMENTS; BINDING PROTEIN; NANOS; TRANSLATION; MICRORNAS; PUMILIO; POLYADENYLATION;

D O I：

10.1073/pnas.1112672108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 3' termini of eukaryotic mRNAs influence transcript stability, translation efficiency, and subcellular localization. Here we report that a subset of developmental regulatory genes, enriched in critical RNA-processing factors, exhibits synchronous lengthening of their 3' UTRs during embryogenesis. The resulting UTRs are up to 20-fold longer than those found on typical Drosophila mRNAs. The large mRNAs emerge shortly after the onset of zygotic transcription, with several of these genes acquiring additional, phased UTR extensions later in embryogenesis. We show that these extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon documented in this study. These findings suggest a previously unknown mode of posttranscriptional regulation that may contribute to the complexity of neurogenesis or neural function.

引用

页码：15864 / 15869

页数：6

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