A novel binary expression vector for production of human IL-10 in Escherichia coli and Bifidobacterium longum

被引：28

作者：

Escogido, M. L. Reyes ^{[1
]}

Rodriguez, A. De Leon ^{[1
]}

de la Rosa, A. P. Barba ^{[1
]}

机构：

[1] Inst Sci & Technol Res San Luis Potosi, Div Mol Biol, San Luis Potosi 78216, SLP, Mexico

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 08期

关键词：

cytokine; delivery systems; protein expression; synthetic gene; sec pathway;

D O I：

10.1007/s10529-007-9376-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.

引用

页码：1249 / 1253

页数：5

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