Construction of a human cardiovascular cDNA microarray: Portrait of the failing heart

被引：62

作者：

Barrans, JD ^{[1
]}

Stamatiou, D

Liew, CC

机构：

[1] Harvard Univ, Brigham & Womens Hosp, Sch Med, Cardiovasc Genome Unit, Boston, MA 02115 USA

[2] Univ Toronto, Toronto Gen Hosp, Univ Hlth Network, Dept Lab Med & Pathobiol, Toronto, ON M5G 1L7, Canada

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 280卷 / 04期

基金：

英国医学研究理事会;

关键词：

cDNA microarray; gene expression; heart failure;

D O I：

10.1006/bbrc.2000.4137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Identifying key genes that regulate the complex diseases of the cardiovascular system can be greatly facilitated with the use of microarrays. In an effort to obtain a global portrait of gene expression in the failing heart, we have constructed in-house a glass microscope slide cDNA microarray (termed "CardioChip") containing 10,368 redundant and randomly-selected sequenced expressed sequence tags (representing known genes, other matched ESTs, and novel, unmatched ESTs) derived from several human heart and artery cDNA libraries. From our preliminary data with Cy3- and Cy5-labeled probes, we have identified 38 transcripts showing a minimum twofold differential expression, among which are several novel or previously-uncharacterized genes. This array-representing what we believe to be the largest cardiovascular-based cDNA array to date-establishes a practical and invaluable platform for obtaining a global genetic portrait of complex cardiovascular diseases, particularly in the failing heart. (C) 2001 Academic Press.

引用

页码：964 / 969

页数：6

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