Single-molecule detection of DNA hybridization

被引：78

作者：

Singh-Zocchi, M ^{[1
]}

Dixit, S ^{[1
]}

Ivanov, V ^{[1
]}

Zocchi, G ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90095 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 13期

关键词：

D O I：

10.1073/pnas.1337215100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We demonstrate the detection of nanometer-scale conformational changes of single DNA oligomers through a micromechanical technique. The quantity monitored is the displacement of a micrometer-size bead tethered to a surface by the probe molecule undergoing the conformational change. This technique allows probing of conformational changes within distances beyond the range of fluorescence resonance energy transfer. We apply the method to detect single hybridization events of label-free target oligomers. Hybridization of the target is detected through the conformational change of the probe.

引用

页码：7605 / 7610

页数：6

共 35 条

[1] Thermodynamic basis of the enhanced specificity of structured DNA probes [J].

Bonnet, G ;

Tyagi, S ;

Libchaber, A ;

Kramer, FR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6171-6176

[2] DNA: An extensible molecule [J].

Cluzel, P ;

Lebrun, A ;

Heller, C ;

Lavery, R ;

Viovy, JL ;

Chatenay, D ;

Caron, F .

SCIENCE, 1996, 271 (5250) :792-794

[3] Stretching single stranded DNA, a model polyelectrolyte [J].

Dessinges, MN ;

Maier, B ;

Zhang, Y ;

Peliti, M ;

Bensimon, D ;

Croquette, V .

PHYSICAL REVIEW LETTERS, 2002, 89 (24)

[4] Single-mismatch detection using gold-quenched fluorescent oligonucleotides [J].

Dubertret, B ;

Calame, M ;

Libchaber, AJ .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :365-370

[5] Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].

Elghanian, R ;

Storhoff, JJ ;

Mucic, RC ;

Letsinger, RL ;

Mirkin, CA .

SCIENCE, 1997, 277 (5329) :1078-1081

[6] Mechanical separation of the complementary strands of DNA [J].

EssevazRoulet, B ;

Bockelmann, U ;

Heslot, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (22) :11935-11940

[7] A fiber-optic DNA biosensor microarray for the analysis of gene expression [J].

Ferguson, JA ;

Boles, TC ;

Adams, CP ;

Walt, DR .

NATURE BIOTECHNOLOGY, 1996, 14 (13) :1681-1684

[8] Electrostatic surface plasmon resonance: Direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches [J].

Heaton, RJ ;

Peterson, AW ;

Georgiadis, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (07) :3701-3704

[9]

ISRAELACHVILI J, 1991, INTREMOLECULAR SURFA

[10] SPECIES-SPECIFIC LONG-RANGE INTERACTIONS BETWEEN RECEPTOR/LIGAND PAIRS [J].

LIEBERT, RB ;

PRIEVE, DC .

BIOPHYSICAL JOURNAL, 1995, 69 (01) :66-73

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