Cysticercosis: Identification and cloning of protective recombinant antigens

被引：64

作者：

Manoutcharian, K ^{[1
]}

Rosas, G ^{[1
]}

Hernandez, M ^{[1
]}

Fragoso, G ^{[1
]}

Aluja, A ^{[1
]}

Villalobos, N ^{[1
]}

Rodarte, LF ^{[1
]}

Sciutto, E ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MEXICO,INST INVEST BIOMED,MEXICO CITY 04510,DF,MEXICO

来源：

JOURNAL OF PARASITOLOGY | 1996年 / 82卷 / 02期

关键词：

D O I：

10.2307/3284156

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We describe the cloning and the evaluation of the protective capacity of 5 recombinant antigens expressed during the cysticercus stage of both Taenia crassiceps and Taenia solium. A cDNA library was constructed in bacteriophage lambda ZAP using mRNA isolated from larvae of T. crassiceps of the ORF strain. The recombinant phage library was screened with polyclonal antibodies against 56- and 74-kDa protective antigen fractions. This screening identified 13 recombinant clones, 5 of which were also strongly recognized by pooled sera from pigs experimentally infected with T. solium. The native antigens are proteins of 56 (clones KETc1, 4, 7) and 74 and 78 kDa (clones KETc11, 12) of T. crassiceps cysticerci. Vaccination experiments using these 5 recombinant clones against murine cysticercosis point to the relevance of KETc1, 4, 7, and 12 in host protection, whereas immunization with the clone KETc11 does not modify the parasite load in females and facilitates the parasitosis in males. We report here the DNA and the deduced amino acid sequence (100 amino acids) of the first protective antigen (KETc7) of potential interest for T. solium pig cysticercosis prevention.

引用

页码：250 / 254

页数：5

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