Identification and characterization of a Zea mays line carrying a transposon-tagged ENOD40

被引:15
作者
Compaan, B
Ruttink, T
Albrecht, C
Meeley, R
Bisseling, T
Franssen, H
机构
[1] Wageningen Univ, Mol Biol Lab, NL-6703 HA Wageningen, Netherlands
[2] Pioneer HiBred Int Inc, Trait & Technol Dev, Iowa City, IA 50310 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2003年 / 1629卷 / 1-3期
关键词
zea mays; mycorrhizal fungi; ENOD40;
D O I
10.1016/j.bbaexp.2003.08.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In Zea mays, two ENOD40 homologous were identified that show only 30% of sequence homology to each other. We identified line e40-mum1 carrying a Mu transposon inserted in ZmENOD40-1, the maize gene that has the highest homology to leguminous ENOD40. The insertion causes a dramatic reduction of the ZmENOD40-1 transcript level. Irrespective of this, homozygous e40-mum1 plants are still able to interact with mycorrhizal fungi. Furthermore, no phenotypic aberrations correlated to the presence of e40-mum1 have been identified and therefore it is suggested that Z mays ENOD40 genes are functionally redundant despite their strikingly low homology. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:84 / 91
页数:8
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