Simultaneous analysis of protoberberine, indolequinoline and quinolone alkaloids in coptis-evodia herb couple and the Chinese herbal preparations by high-performance liquid chromatography-electrospray mass spectrometry

被引：46

作者：

Luo, XB

Chen, B

Yao, SZ ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biol Sensing & Chemometr, Changsha 410082, Peoples R China

[2] Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China

来源：

TALANTA | 2005年 / 66卷 / 01期

关键词：

protoberberine alkaloids; indolequinoline alkaloids; quinolone alkaloids; HPLC-ESI-MS;

D O I：

10.1016/j.talanta.2004.10.006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new and sensitive method has been developed for analysis of protoberberine, indolequinoline and quinolone alkaloids in coptis-evodia herb couple and the Chinese herbal preparations by using HPLC in combination with electrospray mass spectrometry (ESI-MS). Different LC and cone voltages have been optimized prior to this in order to obtain better results and sensitivity. The method was carried out by using a spherigel C-18 analytical column with a gradient solvent system of acetate buffer-acetonitrile-methanol. The contents of alkaloids were measured by pseudomolecule ion on the selective ion recording (SIR) mode. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 9-30 pg. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：103 / 110

页数：8

共 42 条

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