Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography

被引：45

作者：

Kwon, Donghoon ^{[1
]}

Lee, Sanghee ^{[1
]}

Ahn, Myung Mo ^{[1
]}

Kang, In Seok ^{[1
]}

Park, Ki-Hwan ^{[2
]}

Jeon, Sangmin ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Chem Engn, Pohang, South Korea

[2] Chung Ang Univ, Dept Food Sci & Technol, Seoul 156756, South Korea

来源：

ANALYTICA CHIMICA ACTA | 2015年 / 883卷

基金：

新加坡国家研究基金会;

关键词：

Magnetophoretic chromatography; Pathogenic bacteria; Platinum nanoparticles; Colorimetric detection; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; BIOSENSOR; IMMOBILIZATION; IMMUNOSENSOR; STRATEGIES; ASSAY;

D O I：

10.1016/j.aca.2015.04.044

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：61 / 66

页数：6

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