A novel biosensor for specific determination of hydrogen peroxide:: catalase enzyme electrode based on dissolved oxygen probe

A biosensor for the specific determination of hydrogen peroxide was developed using catalase (EC 1.11.1.6) in combination with a dissolved oxygen probe. Catalase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane served as enzyme electrode. The electrode response was maximum when 50 mM phosphate buffer was used at pH 7.0 and at 35 degrees C. The biosensor response depends linearly on hydrogen peroxide concentration between 1.0 x 10(-5) and 3.0 x 10(-3) M with a. response time of 30 s. The sensor is stable for >3 months so in this period >400 assays can be performed. (C) 1999 Elsevier Science B.V. All rights reserved.