MicroRNA-146a regulates the maturation process and pro-inflammatory cytokine secretion by targeting CD40L in oxLDL-stimulated dendritic cells

被引：111

作者：

Chen, Ting ^{[1
]}

Li, Zhoubin ^{[3
]}

Jing, Tu ^{[3
]}

Zhu, Weiguo ^{[1
]}

Ge, Junhua ^{[1
]}

Zheng, Xiaoye ^{[1
]}

Pan, Xiaoping ^{[2
]}

Yan, Hui ^{[1
]}

Zhu, Jianhua ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Cardiol, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China

[3] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Cardiothorac Surg, Hangzhou 310003, Zhejiang, Peoples R China

来源：

FEBS LETTERS | 2011年 / 585卷 / 03期

基金：

中国国家自然科学基金;

关键词：

microRNA-146a; oxLDL; Dendritic cell; CD40L; Immuno-inflammation; CD40-TRAF6; AXIS; HUMAN HEART; EXPRESSION; MACROPHAGES; MECHANISM; LUPUS; PLAY;

D O I：

10.1016/j.febslet.2011.01.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether miRNAs are associated with dendritic cell (DC) immuno-inflammatory responses to oxidized low density lipoprotein (oxLDL) stimulation is yet unknown. Our study aims to explore the link of miRNA to lipid-overload and the immuno-inflammatory mechanism for atherosclerosis. Human primary monocyte-derived DCs were transfected with miR-146a mimics and inhibitor, and then stimulated by oxLDL. For the flow cytometric analysis of the DC immunophenotype, supernatants were collected to determine inflammatory chemokine markers. Our study clearly revealed that miRNA-146a regulates the maturation process and pro-inflammatory cytokine secretion in DCs by targeting CD40L in ox-LDL-stimulated DCs. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：567 / 573

页数：7

共 33 条

[1]

The CD40-TRAF6 axis controls affinity maturation and the generation of long-lived plasma cells [J].