Efficacy of polycationic peptides in preventing vascular graft infection due to Staphylococcus epidermidis

被引:20
作者
Giacometti, A
Cirioni, O
Ghiselli, R
Goffi, L
Mocchegiani, F
Riva, A
Scalise, G
Saba, V
机构
[1] Univ Ancona, INRCA IRCCS, Inst Infect Dis & Publ Hlth, Ancona, Italy
[2] Univ Ancona, INRCA IRCCS, Dept Gen Surg, Ancona, Italy
关键词
D O I
10.1093/jac/46.5.751
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A rat model was used to investigate the efficacy of two polycationic peptides, ranalexin and buforin II, in the prevention of vascular prosthetic graft infection due to methicillin-resistant Staphylococcus epidermidis with intermediate resistance to glycopeptides, The in vitro activity of the peptides was compared with those of vancomycin and teicoplanin by MIC determination and time-kill study. Moreover, the efficacy of collagen-sealed peptide-soaked Dacron was evaluated in a rat model of graft infection. Graft infections were established in the dorsal subcutaneous tissue of 120 adult male Wistar rats. The in vivo study included a control group, one contaminated group that did not receive any antibiotic prophylaxis and four contaminated groups that received an antibiotic-soaked graft. Experiments demonstrated that the activities of buforin II and ranalexin were greater than those of vancomycin and teicoplanin. Particularly, rats with buforin ii-coated Dacron grafts showed no evidence of staphylococcal infection while, for the rats with ranalexin-, vancomycin- and teicoplanin-coated Dacron grafts, the quantitative graft cultures demonstrated bacterial growth (1.9 x 10(2) +/- 0.6 x 10(2) cfu/ml, 6.2 x 10(3) +/- 1.9 x 10(3) cfu/ml and 5.1 x 10(4) +/- 4.8 x 10(3) cfu/ml, respectively), The study demonstrated that the use of peptide-soaked Dacron graft can result in significant bacterial growth inhibition and indicates that these compounds may be potentially useful in prosthetic surgery.
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页码:751 / 756
页数:6
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