Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus

被引：8

作者：

Qiu, MF

Wang, J

Wang, HX

Chen, ZL

Dai, EH

Guo, ZB

Wang, XY

Pang, X

Fan, BX

Wen, J

Wang, J

Yang, RF

机构：

[1] Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

[2] Peoples Liberat Army Gen Hosp, Beijing, Peoples R China

[3] Chinese Acad Sci, Beijing Genom Inst, Beijing, Peoples R China

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2005年 / 12卷 / 03期

关键词：

D O I：

10.1128/CDLI.12.3.474-476.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.

引用

页码：474 / 476

页数：3

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