Simple and rapid method for cis- and trans-resveratrol and piceid isomers determination in wine by high-performance liquid chromatography using Chromolith columns

被引:119
作者
Vian, MA
Tomao, V
Gallet, S
Coulomb, PO
Lacombe, JM
机构
[1] Univ Avignon & Pays Vaucluse, Lab Chim Analyt, UMR A 408, Fac Sci, F-84000 Avignon, France
[2] SARL Engima, Dept Anal Biochim Hameau St Veran, F-84190 Beaumes De Venise, France
关键词
resveratrol; piceid; monolithic column; phytoalexin; HPLC;
D O I
10.1016/j.chroma.2005.05.083
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A simple and rapid HPLC method has been developed for simultaneous determination of the four resveratrol forms (aglycon and glycosidic) in a Grenache wine from Chateauneuf du Pape (Vaucluse). These analyses were achieved by using two commercial monolith HPLC columns and diode array detection. The method provided reliable separations at low pressure with a short analysis time. The limit of detection (LD) and limit of quantification (LQ) were calculated for each standard. The molecules were separated and quantified in a single run without any purification of the sample. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:224 / 229
页数:6
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