Atomic force microscopy:: a powerful tool to observe biomolecules at work

被引：101

作者：

Engel, A

Lyubchenko, Y

Müller, D

机构：

[1] Univ Basel, Biozentrum, ME Muller Inst Microscop Struct Biol, CH-4056 Basel, Switzerland

[2] Arizona State Univ, Dept Microbiol, Tempe, AZ 85287 USA

来源：

TRENDS IN CELL BIOLOGY | 1999年 / 9卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0962-8924(98)01415-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Atomic force microscopes (AFMs) move a sharp tip attached to a soft cantilever in a TV-raster-like pattern over a surface and record deflections of the tip that correspond to the surface topography. When operated in physiological solutions, an AFM allows biomolecules to be observed in their native environment Progress in instrumentation, sample-preparation methods and recording conditions has provided images of biomolecules and their assemblies that reveal submolecular details. In addition the AFM allows conformational changes to be observed directly. This article discusses these points and illustrates them with some pertinent examples.

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页码：77 / 80

页数：4

相关论文

共 37 条

[1] Phase imaging of moving DNA molecules and DNA molecules replicated in the atomic force microscope [J].

Argaman, M ;

Golan, R ;

Thomson, NH ;

Hansma, HG .

NUCLEIC ACIDS RESEARCH, 1997, 25 (21) :4379-4384

[2] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[3] 7X7 RECONSTRUCTION ON SI(111) RESOLVED IN REAL SPACE [J].

BINNIG, G ;

ROHRER, H ;

GERBER, C ;

WEIBEL, E .

PHYSICAL REVIEW LETTERS, 1983, 50 (02) :120-123

[4] 2-DIMENSIONAL CRYSTALLIZATION OF PROTEINS ON PLANAR LIPID FILMS AND STRUCTURE DETERMINATION BY ELECTRON CRYSTALLOGRAPHY [J].

BRISSON, A ;

OLOFSSON, A ;

RINGLER, P ;

SCHMUTZ, M ;

STOYLOVA, S .

BIOLOGY OF THE CELL, 1994, 80 (2-3) :221-228

[5] Scanning force microscopy under aqueous solutions [J].

Bustamante, C ;

Rivetti, C ;

Keller, DJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (05) :709-716

[6] IMAGING THE MEMBRANE-PROTEIN BACTERIORHODOPSIN WITH THE ATOMIC FORCE MICROSCOPE [J].

BUTT, HJ ;

DOWNING, KH ;

HANSMA, PK .

BIOPHYSICAL JOURNAL, 1990, 58 (06) :1473-1480

[7] Analysis of various sequence-specific triplexes by electron and atomic force microscopies [J].

Cherny, DI ;

Fourcade, A ;

Svinarchuk, F ;

Nielsen, PE ;

Malvy, C ;

Delain, E .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :1015-1023

[8] Staphylococcal α-hemolysin can form hexamers in phospholipid bilayers [J].

Czajkowsky, DM ;

Sheng, ST ;

Shao, ZF .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 276 (02) :325-330

[9] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE [J].

DRAKE, B ;

PRATER, CB ;

WEISENHORN, AL ;

GOULD, SAC ;

ALBRECHT, TR ;

QUATE, CF ;

CANNELL, DS ;

HANSMA, HG ;

HANSMA, PK .

SCIENCE, 1989, 243 (4898) :1586-1589

[10] High resolution imaging of native biological sample surfaces using scanning probe microscopy [J].

Engel, A ;

Schoenenberger, CA ;

Muller, DJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (02) :279-284

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