Determination of nucleic acids based on their resonance light scattering enhancement effect on metalloporphyrin derivatives

被引：33

作者：

Chen, ZG ^{[1
]}

Ding, WF

Liang, YZ

Ren, FL

Han, YL

Liu, JB

机构：

[1] Shantou Univ, Dept Chem, Shantou 515063, Peoples R China

[2] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China

来源：

MICROCHIMICA ACTA | 2005年 / 150卷 / 01期

关键词：

resonance light scattering (RLS); metalloporphyrin derivatives; nucleic acids;

D O I：

10.1007/s00604-005-0338-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for the determination of nucleic acids at nanogram per mL level is proposed based on the enhanced resonance light scattering (RLS) signal resulting from the interaction of metalloporphyrins with nucleic acids. Under optimum conditions, the weak RLS signal of metalloporphyrin is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids. The detection limits of calf thymus DNA were 3.5 ng mL(-1), 2.9ng mL(-1) and 1.0 ng mL(-1) for three metalloporphyrins, respectively. Synthetic samples were determined with satisfactory results.

引用

页码：35 / 42

页数：8

共 39 条

[21] Determination for micro amounts of nucleic acids by a resonance light scattering technique with dequalinium chloride [J].

Li, ZP ;

Li, KA ;

Tong, SY .

TALANTA, 2001, 55 (04) :669-675

[22] Resonance double light scattering method for the determination of nucleic acids with cetylpyridine bromide [J].

Liu, RT ;

Yang, JH ;

Sun, CX ;

Wu, X ;

Gao, XB ;

Liu, Y ;

Liu, SF ;

Su, BY .

MICROCHIMICA ACTA, 2004, 147 (1-2) :105-109

[23]

Liu SP, 1999, ANAL CHIM ACTA, V379, P53

[24] Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes [J].

Liu, SP ;

Luo, HQ ;

Li, NB ;

Liu, ZF ;

Zheng, WX .

ANALYTICAL CHEMISTRY, 2001, 73 (16) :3907-3914

[25] PORPHYRIN ASSEMBLIES ON DNA AS STUDIED BY A RESONANCE LIGHT-SCATTERING TECHNIQUE [J].

PASTERNACK, RF ;

BUSTAMANTE, C ;

COLLINGS, PJ ;

GIANNETTO, A ;

GIBBS, EJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (13) :5393-5399

[26] RESONANCE LIGHT-SCATTERING-STUDIES OF PORPHYRIN DIACID AGGREGATES [J].

PASTERNACK, RF ;

SCHAEFER, KF ;

HAMBRIGHT, P .

INORGANIC CHEMISTRY, 1994, 33 (09) :2062-2065

[27] RESONANCE LIGHT-SCATTERING - A NEW TECHNIQUE FOR STUDYING CHROMOPHORE AGGREGATION [J].

PASTERNACK, RF ;

COLLINGS, PJ .

SCIENCE, 1995, 269 (5226) :935-939

[28] EVIDENCE FOR FORMATION OF DNA-BOUND PROTONATED PORPHYRIN ADDUCTS EVEN AT PH-7 [J].

PETHO, G ;

ELLIOTT, NB ;

KIM, MS ;

LIN, MF ;

DIXON, DW ;

MARZILLI, LG .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1993, (20) :1547-1548

[29]

RAGO R, 1990, ANAL BIOCHEM, V191, P31

[30] H-1-NMR STUDIES OF TRIS(PHENANTHROLINE) METAL-COMPLEXES BOUND TO OLIGONUCLEOTIDES - CHARACTERIZATION OF BINDING MODES [J].

REHMANN, JP ;

BARTON, JK .

BIOCHEMISTRY, 1990, 29 (07) :1701-1709

← 1 2 3 4 →