Determination of nucleic acids based on their resonance light scattering enhancement effect on metalloporphyrin derivatives

被引：33

作者：

Chen, ZG ^{[1
]}

Ding, WF

Liang, YZ

Ren, FL

Han, YL

Liu, JB

机构：

[1] Shantou Univ, Dept Chem, Shantou 515063, Peoples R China

[2] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China

来源：

MICROCHIMICA ACTA | 2005年 / 150卷 / 01期

关键词：

resonance light scattering (RLS); metalloporphyrin derivatives; nucleic acids;

D O I：

10.1007/s00604-005-0338-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method for the determination of nucleic acids at nanogram per mL level is proposed based on the enhanced resonance light scattering (RLS) signal resulting from the interaction of metalloporphyrins with nucleic acids. Under optimum conditions, the weak RLS signal of metalloporphyrin is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids. The detection limits of calf thymus DNA were 3.5 ng mL(-1), 2.9ng mL(-1) and 1.0 ng mL(-1) for three metalloporphyrins, respectively. Synthetic samples were determined with satisfactory results.

引用

页码：35 / 42

页数：8

共 39 条

[31] H-1-NMR STUDIES OF TRIS(PHENANTHROLINE) METAL-COMPLEXES BOUND TO OLIGONUCLEOTIDES - STRUCTURAL CHARACTERIZATIONS VIA SELECTIVE PARAMAGNETIC RELAXATION [J].

REHMANN, JP ;

BARTON, JK .

BIOCHEMISTRY, 1990, 29 (07) :1710-1717

[32] PORPHYRIN STUDIES .5. THE METAL COMPLEX SALTS OF ALPHA,BETA,GAMMA,DELTA-TETRAPHENYLPORPHINE [J].

ROTHEMUND, P ;

MENOTTI, AR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1948, 70 (05) :1808-1812

[33] STABLE FLUORESCENT COMPLEXES OF DOUBLE-STRANDED DNA WITH BIS-INTERCALATING ASYMMETRIC CYANINE DYES - PROPERTIES AND APPLICATIONS [J].

RYE, HS ;

YUE, S ;

WEMMER, DE ;

QUESADA, MA ;

HAUGLAND, RP ;

MATHIES, RA ;

GLAZER, AN .

NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2803-2812

[34] USE OF THE DIAMINOBENZOIC ACID FLUORESCENCE ASSAY IN CONJUNCTION WITH UV ABSORBANCE AS A MEANS OF QUANTIFYING AND ASCERTAINING THE PURITY OF A DNA PREPARATION [J].

SCHY, WE ;

PLEWA, MJ .

ANALYTICAL BIOCHEMISTRY, 1989, 180 (02) :314-318

[35] Determination of nucleic acid using the resonance light scattering technique with the mixed complex La(phen)(o-phthalic acid) [J].

Song, GW ;

Cai, ZX ;

Li, L .

MICROCHIMICA ACTA, 2004, 144 (1-3) :23-27

[36] COMPARISON OF THE MOLECULAR AND ELECTRONIC-STRUCTURES OF (MU-OXO)BIS[(5,10,15,20-TETRAPHENYLPORPHYRINATO)IRON(III)] AND (MU-OXO)BIS[(7,8-DIHYDRO-5,10,15,20-TETRAPHENYLPORPHYRINATO)IRON(III)] [J].

STRAUSS, SH ;

PAWLIK, MJ ;

SKOWYRA, J ;

KENNEDY, JR ;

ANDERSON, OP ;

SPARTALIAN, K ;

DYE, JL .

INORGANIC CHEMISTRY, 1987, 26 (05) :724-730

[37] Determination of proteins based on their resonance light scattering enhancement effect on manganese-tetrasulfonatophthalocyanine [J].

Wang, L ;

Li, YX ;

Zhu, CQ ;

Zhao, DH .

MICROCHIMICA ACTA, 2003, 143 (04) :275-279

[38] Determination of yttrium by resonance light scattering technique [J].

Wu, X ;

Li, L ;

Yang, JH ;

Wang, YB ;

Sun, SN ;

Wang, NX .

MICROCHIMICA ACTA, 2003, 141 (3-4) :165-168

[39]

YANG P, 2002, THEORY BIOINORGANIC, P13

← 1 2 3 4 →