Characterisation of the gas-liquid mass transfer in shaking bioreactors

被引:186
作者
Maier, U [1 ]
Büchs, J [1 ]
机构
[1] Aachen Tech Univ, Dept Biochem Engn, Sammelbau Biol, D-52074 Aachen, Germany
关键词
shaking bioreactors; oxygen transfer; screening; process development;
D O I
10.1016/S1369-703X(00)00107-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The maximum gas-liquid mass transfer capacity of 250 ml shaking flasks on orbital shaking machines has been experimentally investigated using the sulphite oxidation method under variation of the shaking frequency, shaking diameter, filling volume and viscosity of the medium. The distribution of the liquid within the flask has been modelled by the intersection between the rotational hyperboloid of the liquid and the inner wall of the shaking flask. This model allows for thr calculation of the specific exchange area (a), the mass transfer coefficient (k(L)) and the maximum oxygen transfer capacity (OTRmax) for given operating conditions and requires no fitting parameters. The model agrees well with the experimental results. It was furthermore shown that the liquid film on the flask wall contributes significantly to the specific mass transfer area (a) and to the oxygen transfer rate (OTR). (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:99 / 106
页数:8
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