p250GAP, a neural RhoGAP protein, is associated with and phosphorylated by Fyn

被引：34

作者：

Taniguchi, S ^{[1
]}

Liu, H ^{[1
]}

Nakazawa, T ^{[1
]}

Yokoyama, K ^{[1
]}

Tezuka, T ^{[1
]}

Yamamoto, T ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Canc Biol, Div Oncol,Minato Ku, Tokyo 1088639, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 306卷 / 01期

关键词：

p250GAP; Fyn; tyrosine phosphorylation; oligodendrocyte; RhoGAP;

D O I：

10.1016/S0006-291X(03)00923-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fyn is a member of the Src-family protein tyrosine kinases and plays important roles in both neurons and oligodendrocytes. Here we report association of Fyn with p250GAP, a RhoGAP protein that is expressed predominantly in brain. p250GAP interacts with Fyn both in vitro and in vivo. p250GAP is tyrosine phosphorylated by Fyn when co-expressed in HEK293T cells. This phosphorylation appears to enhance the interaction between p250GAP and Fyn. Furthermore, the level of tyrosine phosphorylation of p250GAP increases upon differentiation of the oligodendrocyte cell line CG4. Given that Fyn activity is up-regulated during oligodendrocyte maturation, the results argue that p250GAP is phosphorylated by Fyn in oligodendrocytes. Tyrosine phosphorylation of p250GAP by Fyn would regulate its RhoGAP activity, subcellular localization, or interactions with other proteins, leading to morphological and phenotypic changes of oligodendrocytes. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：151 / 155

页数：5

共 25 条

[1] p190 RhoGAP is the principal Src substrate in brain and regulates axon outgrowth, guidance and fasciculation [J].