Raman spectroscopic identification of single yeast cells

被引：86

作者：

Rösch, P ^{[1
]}

Harz, M ^{[1
]}

Schmitt, M ^{[1
]}

Popp, J ^{[1
]}

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2005年 / 36卷 / 05期

关键词：

micro-Raman spectroscopy; yeast cells; single cell identification; eukaryotes; hierarchical cluster analysis;

D O I：

10.1002/jrs.1312

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Eukaryotes (such as yeasts) and prokaryotes (such as bacteria) differ in size, molecular complexity, etc. By definition, eukaryotic cells store their DNA in a separate internal compartment, the so-called nucleus. Owing to this molecular compartmentalization, the identification of yeast cells by vibrational spectroscopy at a single-cell level is challenging. This contribution reports on first results of micro-Raman analysis together with a hierarchical cluster analysis allowing for an online identification method for yeast cells at a single-cell level. For the classification analysis, an average spectrum from 10 different measuring points within a single yeast cell was used to overcome the heterogeneity of the yeast cells. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：377 / 379

页数：5

共 9 条

[1] Yeast identification in the clinical microbiology accepted 15 August 2000 laboratory: phenotypical methods [J].