Analysis of three lupane type triterpenoids in Helicteres angustifolia by high-performance liquid chromatography

被引:20
作者
Chang, YS
Ku, YR
Lin, JH
Lu, KL
Ho, LK
机构
[1] China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan
[2] Natl Labs Foods & Drugs, Dept Hlth, Taipei 115, Taiwan
[3] Chung Hwa Inst Technol, Tainan 717, Taiwan
[4] Natl Yang Ming Univ, Dept & Inst Pharmacol, Taipei 112, Taiwan
关键词
pharmaceutical analysis; Helicteres angustifolia; methyl helicterate;
D O I
10.1016/S0731-7085(01)00465-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Kang-chih-ma is the dried roots and stems of Helicteres angustifolia (Sterculiaceae) and a commonly used folk herbal drug in Taiwan. It possesses anti-dotal, analgesic, anti-inflammatory and anti-bacterial effects and is also known as a kind of tumor inhibitory plant. To evaluate the quality of H. angustifolia, a simple, rapid and accurate high-performance liquid chromatographic (HPLC) method was developed for the assay of three lupane type triterpenes: 3 beta -acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid methyl ester (methyl helicterate), 3 beta -acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid, and 3 beta -acetoxy-27-(p-hydroxyl) benzoyloxylup-20(29)-en-28-oic acid methyl ester. The present HPLC system used an Inertsil ODS-2 column by gradient elution with acetonitrile and water as the mobile phase and detected at UV 230 nm. Regression equations revealed good linear relationships (correlation coefficients: 0.9922-0.9997). The relative standard deviations of these three constituents ranged between 1.05-3.14% (intraday) and 2.12-4.38% (interday). The contents of these three constituents of the heartwood and the bark of the roots of H. angustifolia in five different samples have also been determined. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:849 / 855
页数:7
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