Effects of micro-algae commonly used in aquaculture on acyl-homoserine lactone quorum sensing

被引：84

作者：

Natrah, F. M. I. ^{[1
,2
,3
]}

Kenmegne, Mireille Mardel ^{[1
,2
]}

Wiyoto, Wiyoto ^{[1
,2
]}

Sorgeloos, Patrick ^{[1
,2
]}

Bossier, Peter ^{[1
,2
]}

Defoirdt, Tom ^{[1
,2
]}

机构：

[1] Univ Ghent, Lab Aquaculture, B-9000 Ghent, Belgium

[2] Univ Ghent, Artemia Reference Ctr, B-9000 Ghent, Belgium

[3] Univ Putra Malaysia, Fac Agr, Dept Aquaculture, Serdang 43400, Selangor, Malaysia

来源：

AQUACULTURE | 2011年 / 317卷 / 1-4期

关键词：

Quorum sensing; Micro-algae; Quorum sensing interference; Luminescent vibrios; TO-CELL COMMUNICATION; AHNFELTIOPSIS-FLABELLIFORMIS; GENE-EXPRESSION; VIBRIO-HARVEYI; BACTERIA; ALGA; EXPLOITATION; METABOLITES; INHIBITORS; BIOFILMS;

D O I：

10.1016/j.aquaculture.2011.04.038

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Quorum sensing, bacterial cell-to-cell communication with small signal molecules such as acyl-homoserine lactones, regulates the virulence of many pathogenic bacteria. Therefore, interfering with quorum sensing is currently being explored as a novel biocontrol strategy to fight bacterial infections. In this study, the effects of 19 micro-algal strains on acyl-homoserine lactone-regulated phenotypes of three reporter strains were investigated. Two freshwater micro-algae inhibited violacein production of quorum sensing reporter strain Chromobacterium violaceum CV026. Further tests using Escherichia coli JB523 showed that micro-algal extracts inhibited or stimulated quorum sensing, depending on the algal strain. One freshwater and five marine algae showed quorum sensing inhibitory activity, whereas two algae stimulated quorum sensing-regulated gene expression. Micro-algal strains that showed inhibitory activity in the previous assays also inhibited acylhomoserine lactone-regulated bioluminescence in the aquaculture pathogen Vibrio harveyi. The growth of all reporter strains was found to be unaffected by the micro-algal samples. The most promising micro-algal strain was found to be Chlorella saccharophila CCAP211/48, as its extracts inhibited quorum sensing-regulated gene expression in all three reporter strains. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：53 / 57

页数：5

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