Effects of micro-algae commonly used in aquaculture on acyl-homoserine lactone quorum sensing

被引：84

作者：

Natrah, F. M. I. ^{[1
,2
,3
]}

Kenmegne, Mireille Mardel ^{[1
,2
]}

Wiyoto, Wiyoto ^{[1
,2
]}

Sorgeloos, Patrick ^{[1
,2
]}

Bossier, Peter ^{[1
,2
]}

Defoirdt, Tom ^{[1
,2
]}

机构：

[1] Univ Ghent, Lab Aquaculture, B-9000 Ghent, Belgium

[2] Univ Ghent, Artemia Reference Ctr, B-9000 Ghent, Belgium

[3] Univ Putra Malaysia, Fac Agr, Dept Aquaculture, Serdang 43400, Selangor, Malaysia

来源：

AQUACULTURE | 2011年 / 317卷 / 1-4期

关键词：

Quorum sensing; Micro-algae; Quorum sensing interference; Luminescent vibrios; TO-CELL COMMUNICATION; AHNFELTIOPSIS-FLABELLIFORMIS; GENE-EXPRESSION; VIBRIO-HARVEYI; BACTERIA; ALGA; EXPLOITATION; METABOLITES; INHIBITORS; BIOFILMS;

D O I：

10.1016/j.aquaculture.2011.04.038

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Quorum sensing, bacterial cell-to-cell communication with small signal molecules such as acyl-homoserine lactones, regulates the virulence of many pathogenic bacteria. Therefore, interfering with quorum sensing is currently being explored as a novel biocontrol strategy to fight bacterial infections. In this study, the effects of 19 micro-algal strains on acyl-homoserine lactone-regulated phenotypes of three reporter strains were investigated. Two freshwater micro-algae inhibited violacein production of quorum sensing reporter strain Chromobacterium violaceum CV026. Further tests using Escherichia coli JB523 showed that micro-algal extracts inhibited or stimulated quorum sensing, depending on the algal strain. One freshwater and five marine algae showed quorum sensing inhibitory activity, whereas two algae stimulated quorum sensing-regulated gene expression. Micro-algal strains that showed inhibitory activity in the previous assays also inhibited acylhomoserine lactone-regulated bioluminescence in the aquaculture pathogen Vibrio harveyi. The growth of all reporter strains was found to be unaffected by the micro-algal samples. The most promising micro-algal strain was found to be Chlorella saccharophila CCAP211/48, as its extracts inhibited quorum sensing-regulated gene expression in all three reporter strains. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：53 / 57

页数：5

共 30 条

[11] Regulation of gene expression by cell-to-cell communication: Acyl-homoserine lactone quorum sensing [J].

Fuqua, C ;

Parsek, MR ;

Greenberg, EP .

ANNUAL REVIEW OF GENETICS, 2001, 35 :439-468

[12] Eukaryotic interference with homoserine lactone-mediated prokaryotic signaling [J].

Givskov, M ;

DeNys, R ;

Manefield, M ;

Gram, L ;

Maximilien, R ;

Eberl, L ;

Molin, S ;

Steinberg, PD ;

Kjelleberg, S .

JOURNAL OF BACTERIOLOGY, 1996, 178 (22) :6618-6622

[13] Three parallel quorum-sensing systems regulate gene expression in Vibrio harveyi [J].

Henke, JM ;

Bassler, BL .

JOURNAL OF BACTERIOLOGY, 2004, 186 (20) :6902-6914

[14] Bacterial quorum sensing: Signals, circuits, and implications for biofilms and disease [J].

Jayaraman, Arul ;

Wood, Thomas K. .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2008, 10 :145-167

[15] Cross-kingdom signalling:: exploitation of bacterial quorum sensing molecules by the green seaweed Ulva [J].

Joint, Ian ;

Tait, Karen ;

Wheeler, Glen .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2007, 362 (1483) :1223-1233

[16] Presence of quorum-sensing inhibitor-like compounds from bacteria isolated from the brown alga Colpomenia sinuosa [J].

Kanagasabhapathy, M. ;

Yamazaki, G. ;

Ishida, A. ;

Sasaki, H. ;

Nagata, S. .

LETTERS IN APPLIED MICROBIOLOGY, 2009, 49 (05) :573-579

[17] Quorum sensin inhibitors from the iced alga, Ahnfeltiopsis flabelliformis [J].

Kim, Jung Sun ;

Kim, Yeon Hee ;

Seo, Young Wan ;

Park, Sunghoon .

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2007, 12 (03) :308-311

[18] The effects of betonicine, floridoside, and isethionic acid from the red alga Ahnfeltiopsis flabelliformis on quorum-sensing activity [J].

Liu, Hai Bo ;

Koh, Kyong Pyo ;

Kim, Jung Sun ;

Seo, Youngwan ;

Park, Sunghoon .

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2008, 13 (04) :458-463

[19] Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover [J].

Manefield, M ;

Rasmussen, TB ;

Henzter, M ;

Andersen, JB ;

Steinberg, P ;

Kjelleberg, S ;

Givskov, M .

MICROBIOLOGY-SGM, 2002, 148 :1119-1127

[20] Quorum sensing and Chromobacterium violaceum:: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones [J].

McClean, KH ;

Winson, MK ;

Fish, L ;

Taylor, A ;

Chhabra, SR ;

Camara, M ;

Daykin, M ;

Lamb, JH ;

Swift, S ;

Bycroft, BW ;

Stewart, GSAB ;

Williams, P .

MICROBIOLOGY-UK, 1997, 143 :3703-3711

← 1 2 3 →