Chemiluminometric Flow Injection Analysis procedures for the enzymatic determination of L-alanine, α-ketoglutarate and L-glutamate

被引：19

作者：

Janasek, D ^{[1
]}

Spohn, U ^{[1
]}

机构：

[1] Univ Halle, Inst Biotechnol, D-06120 Halle, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 1999年 / 14卷 / 02期

关键词：

Enzymatic Flow Injection Analysis; chemiluminescence detection; L-alanine; alpha-ketoglutarate; L-glutamate;

D O I：

10.1016/S0956-5663(98)00115-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Chemiluminometric Flow Injection Analysis (FIA) procedures were developed for the enzymatic determination of L-alanine, alpha-ketoglutarate and L-glutamate in the cultivation medium of mammalian cells. A packed bed flow microreactor containing alanine aminotransferase and glutamate oxidase immobilized on sieved porous glass beads was combined with a chemiluminescence detector for the generated hydrogen peroxide. To catalyse the indicator reaction between luminol and hydrogen peroxide both Co(II) ions and immobilized peroxidase from Arthromyces ramosus were used in a fibre optic detector cell. L-alanine, alpha-ketoglutarate and L-glutamate can be detected over two decades of concentration magnitudes with detection limits of 2, 5 and 1 mu M, respectively. The FIA procedure was applied to determine L-alanine and a-ketoglutarate in cell cultivation media. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：123 / 129

页数：7

共 24 条

[11] Immobilization of L-glutamate oxidase and peroxidase for glutamate determination in flow injection analysis system [J].