Maize streak virus-resistant transgenic maize: a first for Africa

被引：44

作者：

Shepherd, Dionne N.

Mangwende, Tichaona

Martin, Darren P.

Bezuidenhout, Marion

Kloppers, Frederik J.

Carolissen, Charlene H.

Monjane, Aderito L.

Rybicki, Edward P.

Thomson, Jennifer A.

机构：

[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa

[2] Univ Cape Town, Dept Pharmacol, ZA-7925 Cape Town, South Africa

[3] Univ Cape Town, Inst Infect Dis & Mol Med, ZA-7925 Cape Town, South Africa

[4] PANNAR PTY Ltd, ZA-3250 Greytown, South Africa

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2007年 / 5卷 / 06期

关键词：

African biotechnology; geminivirus; maize streak virus; maize streak virus-resistant maize; maize yield improvement; pathogen-derived resistance; transgenic maize;

D O I：

10.1111/j.1467-7652.2007.00279.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this article, we report transgene-derived resistance in maize to the severe pathogen maize streak virus (MSV). The mutated MSV replication-associated protein gene that was used to transform maize showed stable expression to the fourth generation. Transgenic T-2 and T-3 plants displayed a significant delay in symptom development, a decrease in symptom severity and higher survival rates than non-transgenic plants after MSV challenge, as did a transgenic hybrid made by crossing T-2 Hi-II with the widely grown, commercial, highly MSV-susceptible, white maize genotype WM3. To the best of our knowledge, this is the first maize to be developed with transgenic MSV resistance and the first all-African-produced genetically modified crop plant.

引用

页码：759 / 767

页数：9

共 42 条

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