Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

被引：45

作者：

Ayyadurai, Niraikulam ^{[1
]}

Prabhu, Nadarajan Saravanan ^{[1
]}

Deepankumar, Kanagavel ^{[1
]}

Jang, Yoon Jung ^{[2
]}

Chitrapriya, Nataraj ^{[2
]}

Song, Eunjung ^{[3
]}

Lee, Nahum ^{[3
]}

Kim, Seog K. ^{[2
]}

Kim, Byung-Gee ^{[3
]}

Soundrarajan, Nagasundarapandian ^{[4
]}

Lee, Sungu ^{[4
]}

Cha, Hyung Joon ^{[5
]}

Budisa, Nediljko ^{[6
]}

Yun, Hyungdon ^{[1
]}

机构：

[1] Yeungnam Univ, Sch Biotechnol, Gyongsan, South Korea

[2] Yeungnam Univ, Dept Chem, Gyongsan, South Korea

[3] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul, South Korea

[4] Pusan Natl Univ, Dept Chem Engn, Pusan, South Korea

[5] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang, South Korea

[6] Tech Univ Berlin, Dept Biocatalysis, D-1000 Berlin, Germany

来源：

BIOCONJUGATE CHEMISTRY | 2011年 / 22卷 / 04期

关键词：

CHEMISTRY;

D O I：

10.1021/bc2000066

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation of 3,4-dihydroxy-L-phenylalanine can alter the properties of protein to conjugate into the polymers. The homogeneously modified protein will yield quinone residues that are covalently conjugated to nucleophilic groups of the amino polysaccharide. This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications.

引用

页码：551 / 555

页数：5

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