Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in enterococci

被引：75

作者：

Sinclair, A

Arnold, C

Woodford, N

机构：

[1] Hlth Protect Agcy Colindale, SRMD, ARMRL, London NW9 5HT, England

[2] Hlth Protect Agcy Colindale, SRMD, Genom Proteom & Bioinformat Unit, London NW9 5HT, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2003年 / 47卷 / 11期

关键词：

D O I：

10.1128/AAC.47.11.3620-3622.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either 62576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

引用

收藏

页码：3620 / 3622

页数：3

相关论文

共 13 条

[1] Linezolid-resistant enterococci: report of the first isolates in the United Kingdom [J].

Auckland, C ;

Teare, L ;

Cooke, F ;

Kaufmann, ME ;

Warner, M ;

Jones, G ;

Bamford, K ;

Ayles, H ;

Johnson, AP .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 50 (05) :743-746

[2] A novel method for preparing single-stranded DNA for Pyrosequencing™ [J].

Diggle, MA ;

Clarke, SC .

MOLECULAR BIOTECHNOLOGY, 2003, 24 (02) :221-224

[3] Determination of hepatitis C virus genotype by Pyrosequencing [J].

Elahi, E ;

Pourmand, N ;

Chaung, R ;

Rofoogaran, A ;

Boisver, J ;

Samimi-Rad, K ;

Davis, RW ;

Ronaghi, M .

JOURNAL OF VIROLOGICAL METHODS, 2003, 109 (02) :171-176

[4]

Ford C. W., 2001, Current Drug Targets - Infectious Disorders, V1, P181, DOI 10.2174/1568005014606099

[5] Emerging linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolated from two Austrian patients in the same intensive care unit [J].

Johnson, AP ;

Tysall, L ;

Stockdale, MW ;

Woodford, N ;

Kaufmann, ME ;

Warner, M ;

Livermore, DM ;

Asboth, F ;

Allerberger, FJ .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2002, 21 (10) :751-754

[6] Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis [J].

Marshall, SH ;

Donskey, CJ ;

Hutton-Thomas, R ;

Salata, RA ;

Rice, LB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (10) :3334-3336

[7] Single-nucleotide polymorphism detection in plants using a single-stranded pyrosequencing protocol with a universal biotinylated primer [J].

Pacey-Miller, T ;

Henry, R .

ANALYTICAL BIOCHEMISTRY, 2003, 317 (02) :165-170

[8] Linezolid resistance in Staphylococcus aureus:: Characterization and stability of resistant phenotype [J].

Pillai, SK ;

Sakoulas, G ;

Wennersten, C ;

Eliopoulos, GM ;

Moellering, RC ;

Ferraro, MJ ;

Gold, HS .

JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (11) :1603-1607

[9] Resistance to linezolid: Characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci [J].

Prystowsky, J ;

Siddiqui, F ;

Chosay, J ;

Shinabarger, DL ;

Millichap, J ;

Peterson, LR ;

Noskin, GA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (07) :2154-2156

[10] A sequencing method based on real-time pyrophosphate [J].

Ronaghi, M ;

Uhlén, M ;

Nyrén, P .

SCIENCE, 1998, 281 (5375) :363-+