Supported planar bilayer formation by vesicle fusion: the interaction of phospholipid vesicles with surfaces and the effect of gramicidin on bilayer properties using atomic force microscopy

被引：174

作者：

Leonenko, ZV ^{[1
]}

Carnini, A ^{[1
]}

Cramb, DT ^{[1
]}

机构：

[1] Univ Calgary, Dept Chem, Calgary, AB T2N 1N4, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2000年 / 1509卷 / 1-2期

基金：

加拿大自然科学与工程研究理事会;

关键词：

dioleoylphosphatidylcholine; elasticity; fusion; size distribution; bilayer;

D O I：

10.1016/S0005-2736(00)00288-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used magnetic alternating current mode atomic force microscopy (MAC-AFM) to investigate the formation of supported phospholipid bilayers (SPB) by the method of vesicle fusion. The systems studied were dioleoylphosphatidylcholine (DOPC) on mica and mica modified with 3-aminopropyl-triethoxy-silane (APTES), and DOPC vesicles with gramicidin incorporated on mica and APTES-modified mica. The AFM images reveal three stages of bilayer formation: localized disklike features that are single bilayer footprints of the vesicles, partial continuous coverage, and finally complete bilayer formation. The mechanism of supported phospholipid bilayers formation is the fusion of proximal vesicles, rather than surface disk migration. This mechanism does not appear to be affected by incorporation of gramicidin or by surface modification. Once formed, the bilayer develops circular defects one bilayer deep. These defects grow in size and number until a dynamic equilibrium is reached. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：131 / 147

页数：17

共 48 条

[1] Manipulation and molecular resolution of a phosphatidylcholine-supported planar bilayer by atomic force microscopy [J].