Galectin-1 is expressed in early-type neural progenitor cells and down-regulates neurogenesis in the adult hippocampus

被引：24

作者：

Imaizumi, Yoichi ^{[1
,2
]}

Sakaguchi, Masanori ^{[1
]}

Morishita, Tsuyoshi ^{[3
]}

Ito, Mamoru

Poirier, Francoise ^{[4
]}

Sawamoto, Kazunobu ^{[2
,5
]}

Okano, Hideyuki ^{[1
]}

机构：

[1] Keio Univ, Sch Med, Dept Physiol, Tokyo 160, Japan

[2] Keio Univ, Sch Med, Bridgestone Lab Dev & Regenerat Neurobiol, Tokyo, Japan

[3] Kyowa Hakko Kirin Co Ltd, Dev Div, Tokyo, Japan

[4] Univ Paris Diderot, CNRS, UMR 7592, Inst Jacques Monod, Paris, France

[5] Nagoya City Univ, Grad Sch Med Sci, Dept Dev & Regenerat Biol, Nagoya, Aichi, Japan

来源：

MOLECULAR BRAIN | 2011年 / 4卷

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

CENTRAL-NERVOUS-SYSTEM; STEM-CELLS; SUBVENTRICULAR ZONE; BINDING PROTEIN; DENTATE GYRUS; GENETIC INFLUENCE; FOREBRAIN; MICE; MOUSE; DIFFERENTIATION;

D O I：

10.1186/1756-6606-4-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Background: In the adult mammalian brain, neural stem cells (NSCs) proliferate in the dentate gyrus (DG) of the hippocampus and generate new neurons throughout life. A multimodal protein, Galectin-1, is expressed in neural progenitor cells (NPCs) and implicated in the proliferation of the NPCs in the DG. However, little is known about its detailed expression profile in the NPCs and functions in adult neurogenesis in the DG. Results: Our immunohistochemical and morphological analysis showed that Galectin-1 was expressed in the type 1 and 2a cells, which are putative NSCs, in the subgranular zone (SGZ) of the adult mouse DG. To study Galectin-1's function in adult hippocampal neurogenesis, we made galectin-1 knock-out mice on the C57BL6 background and characterized the effects on neurogenesis. In the SGZ of the galectin-1 knock-out mice, increased numbers of type 1 cells, DCX-positive immature progenitors, and NeuN-positive newborn neurons were observed. Using triple-labeling immunohistochemistry and morphological analyses, we found that the proliferation of the type-1 cells was increased in the SGZ of the galectin-1 knock-out mice, and we propose that this proliferation is the mechanism for the net increase in the adult neurogenesis in these knock-out mice DG. Conclusions: Galectin-1 is expressed in the neural stem cells and down-regulates neurogenesis in the adult hippocampus.

引用

页数：10