Whispering-gallery-mode biosensing: label-free detection down to single molecules

被引:1368
作者
Vollmer, Frank [2 ]
Arnold, Stephen [1 ]
机构
[1] Polytech Univ, MicroParticle PhotoPhys Lab MPL, Brooklyn, NY 11201 USA
[2] Harvard Univ, Rowland Inst, Cambridge, MA 02142 USA
基金
美国国家科学基金会;
关键词
D O I
10.1038/nmeth.1221
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.
引用
收藏
页码:591 / 596
页数:6
相关论文
共 36 条
[1]   Label-free, single-molecule detection with optical microcavities [J].
Armani, Andrea M. ;
Kulkarni, Rajan P. ;
Fraser, Scott E. ;
Flagan, Richard C. ;
Vahala, Kerry J. .
SCIENCE, 2007, 317 (5839) :783-787
[2]   Soft lithographic fabrication of high Q polymer microcavity arrays [J].
Armani, Andrea M. ;
Srinivasan, Akil ;
Vahala, Kerry J. .
NANO LETTERS, 2007, 7 (06) :1823-1826
[3]   MicroParticle photophysics illuminates viral bio-sensing [J].
Arnold, S. ;
Ramjit, R. ;
Keng, D. ;
Kolchenko, V. ;
Teraoka, I. .
FARADAY DISCUSSIONS, 2008, 137 :65-83
[4]   Shift of whispering-gallery modes in microspheres by protein adsorption [J].
Arnold, S ;
Khoshsima, M ;
Teraoka, I ;
Holler, S ;
Vollmer, F .
OPTICS LETTERS, 2003, 28 (04) :272-274
[5]   Microspheres, photonic atoms and the physics of nothing [J].
Arnold, S .
AMERICAN SCIENTIST, 2001, 89 (05) :414-421
[6]   Nanofabrication of optical structures and devices for photonics and biophotonics [J].
Boyd, RW ;
Heebner, JE ;
Lepeshkin, NN ;
Park, QH ;
Schweinsberg, A ;
Wicks, GW ;
Baca, AS ;
Fajardo, JE ;
Hancock, RR ;
Lewis, MA ;
Boysel, RM ;
Quesada, M ;
Welty, R ;
Bleier, AR ;
Treichler, J ;
Slusher, RE .
JOURNAL OF MODERN OPTICS, 2003, 50 (15-17) :2543-2550
[7]   Sensitive disk resonator photonic biosensor [J].
Boyd, RW ;
Heebner, JE .
APPLIED OPTICS, 2001, 40 (31) :5742-5747
[8]   Polymer microring resonators for biochemical sensing applications [J].
Chao, CY ;
Fung, W ;
Guo, LJ .
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2006, 12 (01) :134-142
[9]   Label-free screening of bio-molecular interactions [J].
Cooper, MA .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 377 (05) :834-842
[10]   Silicon-on-Insulator microring resonator for sensitive and label-free biosensing [J].
De Vos, Katrien ;
Bartolozzi, Irene ;
Schacht, Etienne ;
Bienstman, Peter ;
Baets, Roel .
OPTICS EXPRESS, 2007, 15 (12) :7610-7615