Whispering-gallery-mode biosensing: label-free detection down to single molecules

被引：1368

作者：

Vollmer, Frank ^{[2
]}

Arnold, Stephen ^{[1
]}

机构：

[1] Polytech Univ, MicroParticle PhotoPhys Lab MPL, Brooklyn, NY 11201 USA

[2] Harvard Univ, Rowland Inst, Cambridge, MA 02142 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 07期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/nmeth.1221

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical label-free detectors, such as the venerable surface plasmon resonance (SPR) sensor, are generally favored for their ability to obtain quantitative data on intermolecular binding. However, before the recent introduction of resonant microcavities that use whispering gallery mode (WGM) recirculation, sensitivity to single binding events had not materialized. Here we describe the enhancement mechanisms responsible for the extreme sensitivity of the WGM biosensor, review its current implementations and applications, and discuss its future possibilities.

引用

页码：591 / 596

页数：6

共 36 条

[11] Characteristics of dynamic mass redistribution of epidermal growth factor receptor signaling in living cells measured with label-free optical biosensors [J].

Fang, Y ;

Ferrie, AM ;

Fontaine, NH ;

Yuen, PK .

ANALYTICAL CHEMISTRY, 2005, 77 (17) :5720-5725

[12]

Fang Y, 2006, BIOPHYS J, V91, P1925, DOI [10.1529/biophysj.105.077818, 10.1529/biophysj.105.007818]

[13] Morphology-dependent resonances of a microsphere-optical fiber system [J].

Griffel, G ;

Arnold, S ;

Taskent, D ;

Serpenguzel, A ;

Connolly, J ;

Morris, N .

OPTICS LETTERS, 1996, 21 (10) :695-697

[14] Mimicking photosynthetic solar energy transduction [J].

Gust, D ;

Moore, TA ;

Moore, AL .

ACCOUNTS OF CHEMICAL RESEARCH, 2001, 34 (01) :40-48

[15] Conformational changes in G-protein-coupled receptors -: the quest for functionally selective conformations is open [J].

Hoffmann, C. ;

Zuern, A. ;

Buenemann, M. ;

Lohse, M. J. .

BRITISH JOURNAL OF PHARMACOLOGY, 2008, 153 :S358-S366

[16] Resonance fluctuations of a whispering gallery mode biosensor by particles undergoing Brownian motion [J].

Keng, D. ;

McAnanama, S. R. ;

Teraoka, I. ;

Arnold, S. .

APPLIED PHYSICS LETTERS, 2007, 91 (10)

[17] Phase-matched excitation of whispering-gallery-mode resonances by a fiber taper [J].

Knight, JC ;

Cheung, G ;

Jacques, F ;

Birks, TA .

OPTICS LETTERS, 1997, 22 (15) :1129-1131

[18] Two-dimensional silicon photonic crystal based biosensing platform for protein detection [J].

Lee, Mindy ;

Fauchet, Philippe M. .

OPTICS EXPRESS, 2007, 15 (08) :4530-4535

[19]

McDonald JC, 2000, ELECTROPHORESIS, V21, P27, DOI 10.1002/(SICI)1522-2683(20000101)21:1<27::AID-ELPS27>3.0.CO

[20]

2-C

← 1 2 3 4 →