Proteomics for biodefense applications: progress and opportunities

被引:10
作者
Drake, RR
Deng, YP
Schwegler, EE
Gravenstein, S
机构
[1] Eastern Virginia Med Sch, Sci Ctr Biodef, Ctr Biomed Proteom, Dept Microbiol & Mol Cell Biol, Norfolk, VA 23507 USA
[2] Eastern Virginia Med Sch, Dept Internal Med, Glennan Ctr Geriatr & Gerontol, Norfolk, VA 23507 USA
关键词
2D SDS-PAGE; biodefense; biomarkers; ICAT; immunoproteomics; MALDI; SELDI;
D O I
10.1586/14789450.2.2.203
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The increasing threat of bioterrorism and continued emergence of new infectious diseases has driven a major resurgence in biomedical research efforts to develop improved treatments, diagnostics and vaccines, as well as increase the fundamental understanding of the host immune response to infectious agents. The availability of multiple mass spectrometry platforms combined with multidimensional separation technologies and microbial genomic databases provides an unprecedented opportunity to develop these much needed resources. An overview of current proteomic strategies applied to microbes and viruses considered potential bioterrorism agents is presented. The emerging area of immunoproteomics as applied to the development of new vaccine targets is also summarized. These powerful research approaches can generate a multitude of potential new protein targets; however, translating these proteomic discoveries to useful counter-bioterrorism products will require large collaborative research efforts across multiple basic science and clinical disciplines. A translational proteomic research paradigm illustrating this approach using influenza virus as an example is discussed.
引用
收藏
页码:203 / 213
页数:11
相关论文
共 87 条
[61]   The cell wall subproteome of Listeria monocytogenes [J].
Schaumburg, J ;
Diekmann, O ;
Hagendorff, P ;
Bergmann, S ;
Rohde, M ;
Hammerschmidt, S ;
Jänsch, L ;
Wehland, J ;
Kärst, U .
PROTEOMICS, 2004, 4 (10) :2991-3006
[62]   Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope-coded affinity tag technology [J].
Schmidt, F ;
Donahoe, S ;
Hagens, K ;
Mattow, J ;
Schaible, UE ;
Kaufmann, SHE ;
Aebersold, R ;
Jungblut, PR .
MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (01) :24-42
[63]   SELDI-TOF MS profiling of serum for detection of the progression of chronic hepatitis C to hepatocellular carcinoma [J].
Schwegler, EE ;
Cazares, L ;
Steel, LF ;
Adam, BL ;
Johnson, DA ;
Semmes, OJ ;
Block, TM ;
Marrero, JA ;
Drake, FR .
HEPATOLOGY, 2005, 41 (03) :634-642
[64]  
SEMMES OJ, 2005, IN PRESS CLIN CANC R
[65]   MHC-bound antigens and proteomics for novel target discovery [J].
Shoshan, SH ;
Admon, A .
PHARMACOGENOMICS, 2004, 5 (07) :845-859
[66]  
Skultéty L, 2004, ACTA VIROL, V48, P29
[67]   Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions [J].
Starck, J ;
Källenius, G ;
Marklund, BI ;
Andersson, DI ;
Åkerlund, T .
MICROBIOLOGY-SGM, 2004, 150 :3821-3829
[68]   Identification of the immunodominant protein and other proteins of the Bacillus anthracis exosporium [J].
Steichen, C ;
Chen, P ;
Kearney, JF ;
Turnbough, CL .
JOURNAL OF BACTERIOLOGY, 2003, 185 (06) :1903-1910
[69]   Mortality associated with influenza and respiratory syncytial virus in the United States [J].
Thompson, WW ;
Shay, DK ;
Weintraub, E ;
Brammer, L ;
Cox, N ;
Anderson, LJ ;
Fukuda, K .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2003, 289 (02) :179-186
[70]   Detection and identification of virulence factors in Yersinia pestis using SELDI ProteinChip® system [J].
Thulasiraman, V ;
McCutchen-Maloney, SL ;
Motin, VL ;
Garcia, E .
BIOTECHNIQUES, 2001, 30 (02) :428-432