In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand

被引：17

作者：

Ai, Jun ^{[1
,2
]}

Li, Tao ^{[1
,2
]}

Li, Bingling ^{[1
,2
]}

Xu, Yuanhong ^{[1
,2
]}

Li, Dan ^{[1
,2
]}

Liu, Zuojia ^{[1
,2
]}

Wang, Erkang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2012年 / 741卷

基金：

中国国家自然科学基金;

关键词：

AS1411; Nucleolin; HeLa cells; Fluorescence; Bioimaging; NANOPARTICLES; BINDING; SURFACE; PROBE;

D O I：

10.1016/j.aca.2012.06.048

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：93 / 99

页数：7

共 25 条

[21] SYSTEMATIC EVOLUTION OF LIGANDS BY EXPONENTIAL ENRICHMENT - RNA LIGANDS TO BACTERIOPHAGE-T4 DNA-POLYMERASE [J].