Validation of a rapid microtiter plate assay to conduct cytochrome P450 2D6 enzyme inhibition studies

被引：28

作者：

Palamanda, JR ^{[1
]}

Favreau, L ^{[1
]}

Lin, CC ^{[1
]}

Nomeir, AA ^{[1
]}

机构：

[1] Schering Plough Res Inst, Dept Drug Metab & Pharmacokinet, Kenilworth, NJ 07033 USA

来源：

DRUG DISCOVERY TODAY | 1998年 / 3卷 / 10期

关键词：

D O I：

10.1016/S1359-6446(98)01248-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Cytochrome P450 (CYP) inhibition studies are often performed on new chemical entities during the drug discovery stage. However, advances in combinatorial chemistry mean that an unprecedented number of compounds now require such evaluation. Recently, a microtiter plate assay that utilizes individually expressed CYP enzymes (Supersomes(TM)) has been developed to facilitate this process. The authors discuss some of their experiences with this technology and its potential impact for higher throughput CYP2D6 enzyme inhibition studies.

引用

页码：466 / 470

页数：5

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