Microcalorimetric study of the growth of bacterial colonies of Lactococcus lactis IL1403 in agar gels

被引:44
作者
Kabanova, N. [1 ,2 ]
Stulova, I. [1 ,2 ]
Vilu, R. [1 ,2 ]
机构
[1] Competence Ctr Food & Fermentat Technol CCFFT, EE-12618 Tallinn, Estonia
[2] Tallinn Univ Technol, EE-19086 Tallinn, Estonia
关键词
Colonial growth; LAB; Microcalorimetry; Solid-state fermentations; ISOTHERMAL MICROCALORIMETRY; ACID; CALORIMETRY; METABOLISM; FOOD; PH; MICROSTRUCTURE; THERMODYNAMICS; ENERGETICS; BATCH;
D O I
10.1016/j.fm.2011.08.018
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
Growth of Lactococcus lactis IL1403 in solid agar gels and liquid cultures at different glucose concentrations of 2, 10 and 20 g/L and different inoculation rates from 10(0) to 10(6) cfu/mL with the 10-fold increment was studied using thermal activity monitor TAM III. In parallel to calorimetric measurements the changes of glucose and lactic acid concentrations and pH of culture media were measured in order to obtain additional information for the interpretation of calorimetric power-time curves. Maximal specific growth rates of heat evolution proportional to growth rates of biomass mu(max) (W/h), heat produced during different growth stages Q(TOT) Q(ExP) (J/mL) and duration of lag-phases lambda (h) were obtained by processing calorimetric curves. The sizes of colonies; were measured also at the end of growth using microscope. The data obtained together with calculated heat yield coefficient Y-Q (J/cfu) allowed to analyze and describe quantitatively the growth of individual colonies and develop a model of multistage growth of a typical colony of L. lactis in 1% agar gel. Microcalorimetry used in combination with other relevant methods is a very powerful and precise tool in studying solid-state fermentations. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:67 / 79
页数:13
相关论文
共 46 条
[1]
Accelerated storage and isothermal microcalorimetry as methods of predicting carrot juice shelf-life [J].
Alklint, C ;
Wadsö, L ;
Sjöholm, I .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2005, 85 (02) :281-285
[2]
The metabolic pH response in Lactococcus lactis: An integrative experimental and modelling approach [J].
Andersen, Ann Zahle ;
Carvalho, Ana Lucia ;
Neves, Ana Rute ;
Santos, Helena ;
Kummer, Ursula ;
Olsen, Lars Folke .
COMPUTATIONAL BIOLOGY AND CHEMISTRY, 2009, 33 (01) :71-83
[3]
Influence of a gel microstructure as modified by gelatin concentration on Listeria innocua growth [J].
Antwi, M. ;
Geeraerd, A. H. ;
Vereecken, K. M. ;
Jenne, R. ;
Bernaerts, K. ;
Van Impe, J. F. .
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, 2006, 7 (1-2) :124-131
[5]
UNCOUPLING IN BACTERIAL GROWTH - EFFECT OF PANTOTHENATE STARVATION ON GROWTH OF ZYMOMONAS-MOBILIS [J].
BELAICH, JP ;
BELAICH, A ;
SIMONPIETRI, P .
JOURNAL OF GENERAL MICROBIOLOGY, 1972, 70 (APR) :179-+
[6]
CALORIMETRIC INVESTIGATION OF AEROBIC FERMENTATIONS [J].
BIROU, B ;
MARISON, IW ;
VONSTOCKAR, U .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (05) :650-660
[7]
CELL COUNTING AND CARBON UTILIZATION VELOCITIES VIA MICROBIAL CALORIMETRY [J].
BOE, I ;
LOVRIEN, R .
BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (01) :1-7
[8]
"The heat is on": Rapid microcalorimetric detection of mycobacteria in culture [J].
Braissant, O. ;
Wirz, D. ;
Goepfert, B. ;
Daniels, A. U. .
TUBERCULOSIS, 2010, 90 (01) :57-59
[9]
Use of isothermal microcalorimetry to monitor microbial activities [J].
Braissant, Olivier ;
Wirz, Dieter ;
Goepfert, Beat ;
Daniels, Alma U. .
FEMS MICROBIOLOGY LETTERS, 2010, 303 (01) :1-8
[10]
Guan Y, 1998, BIOTECHNOL BIOENG, V58, P464, DOI 10.1002/(SICI)1097-0290(19980605)58:5<464::AID-BIT2>3.0.CO