IFN-γ but not IL-4 T cells of the asthmatic bronchial wall show increased incidence of apoptosis

被引:21
作者
Cormican, L
O'Sullivan, S
Burke, CM
Poulter, W
机构
[1] UCL Royal Free & Univ Coll Sch Med, Dept Clin Immunol, London NW3 2PF, England
[2] James Connolly Mem Hosp, Dept Resp Med, Dublin, Ireland
关键词
bronchial biopsy; asthma; apoptosis; Bcl-2; TUNEL; IFN-gamma; IL-4;
D O I
10.1046/j.1365-2222.2001.01099.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background Previous observations have established that IFN-gamma production is depressed in CD4(+) T cells from atopic asthmatics compared with non-asthmatics. Objective The aim of this study was to determine if decreased IFN-gamma production could be due to a dissociation between levels of apoptosis within the T cell subsets of the asthmatic bronchial wall. Methods Twenty asthmatics (10 atopic and 10 non-atopic) and eight non-atopic nonasthmatics underwent bronchoscopy. Cryostat sections of these biopsies were investigated using immunohistological techniques to determine the relative number of CD4/FAS+ and CD4/Bcl-2+ cells. Detection of IFN-gamma (+) and IL-4(+) was combined with TUNEL staining to determine the proportions of the Th1 and Th2 cells undergoing apoptosis. Results Experiments revealed raised proportions of activated CD4(+) T cells as assessed by expression of HLA-DR and CD25(+) expression in the asthmatic samples. Expression of Bcl-2 by the CD4(+) cell population was significantly reduced in the asthmatic compared with the control group (P = 0.002). There was mo significant difference in the expression of CD4(+) Fas-ligand or the number of CD4(+) undergoing apoptosis in the asthmatic and non-asthmatic groups. However, the IFN-gamma (+) (P = 0.04) but not IL-4(+) T cells in the asthmatic biopsies had significantly higher proportions of apoptotic cells compared with the control group. Conclusion The evidence supports the hypothesis that Th1/Th2 imbalance in asthmatic inflammation may be a result of premature apoptosis within the Th1 subset.
引用
收藏
页码:731 / 739
页数:9
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