Antioxidant effect of flavonoids on DCF production in HL-60 cells

被引：48

作者：

Takamatsu, S ^{[1
]}

Galal, AM

Ross, SA

Ferreira, D

ElSohly, MA

Ibrahim, ARS

El-Feraly, FS

机构：

[1] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Res Inst Pharmaceut Sci, University, MS 38677 USA

[3] Tanta Univ, Coll Pharm, Dept Pharmacognosy, Tanta 8130, Egypt

[4] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh 11451, Saudi Arabia

来源：

PHYTOTHERAPY RESEARCH | 2003年 / 17卷 / 08期

关键词：

respiratory burst activity; DCFH oxidation; antioxidants; flavonoids; HL-60; cell;

D O I：

10.1002/ptr.1289

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A fluorometric microplate assay was performed for the detection of respiratory burst activity in a human leukaemia cell line HL-60 by assessing oxidation of 2',7'-dichlorofluorescin diacetate (DCFH-DA). This method is based on the detection of DCFH oxidation due to the presence of hydrogen peroxide. In the present study, the antioxidant activity of a number of structurally related flavonoids of plant origin and some of their microbial transformation products (1-18) were evaluated. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：963 / 966

页数：4

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