The simultaneous determination of diazepam and its three metabolites in dog plasma by high-performance liquid chromatography with mass spectroscopy detection

被引：33

作者：

Liu, Z

Short, J

Rose, A

Ren, S

Contel, N

Grossman, S

Unger, S

机构：

[1] DuPont Pharmaceut Co, Stine Haskell Res Ctr, Dept Drug Metab & Pharmacokinet, Newark, DE 19714 USA

[2] TAP Pharmaceut Prod, Lake Forest, IL 60045 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2001年 / 26卷 / 02期

关键词：

HPLC methods; electrospray ionization; MS/MS detection; dog plasma; automated SPE;

D O I：

10.1016/S0731-7085(01)00404-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast, sensitive and specific LC/MS/MS method for the simultaneous determination of diazepam and its three metabolites, oxazepam, temazepam and desmethyldiazepam. in dog plasma is described. The method consists of an automated 96-well solid phase extraction procedure and electrospray LC/MS/MS analysis. D-5-Diazepam is used as the internal standard for all the compounds. Intra-day and inter-day assay coefficients of variations are less than 12.7%. The lower limit of quantitation (LLOQ) is 1 nM for each analyte, based on 0.1 ml aliquots of dog plasma. The analytical run time was 5 min, Linearity is observed over the range of 1-500 nM. This method has been used to support the discovery of pharmacokinetic studies. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：321 / 330

页数：10

共 13 条

[1] DETERMINATION OF 1,4-BENZODIAZEPINES AND 1,4-BENZODIAZEPINE-2-ONES IN BLOOD BY ELECTRON-CAPTURE GAS-LIQUID-CHROMATOGRAPHY [J].