Environment and excavation: Pre-lab impacts on ancient DNA analyses

被引：57

作者：

Bollongino, Ruth ^{[1
,2
]}

Tresset, Anne ^{[1
]}

Vigne, Jean-Denis ^{[1
]}

机构：

[1] Museum Natl Hist Nat, UMR 5197, CNRS, Dept Ecol & Gest Biodiversite, F-75231 Paris 05, France

[2] Johannes Gutenberg Univ Mainz, Inst Anthropol, Palaeogenet Grp, D-55128 Mainz, Germany

来源：

COMPTES RENDUS PALEVOL | 2008年 / 7卷 / 2-3期

关键词：

ancient DNA; contamination; DNA preservation; sampling strategy; molecular genetics; excavation techniques;

D O I：

10.1016/j.crpv.2008.02.002

中图分类号：

Q91 [古生物学];

学科分类号：

0709 ; 070903 ;

摘要：

Ancient DNA (aDNA) analyses enjoy an increasing role in palaeontological, archaeological and archaeozoological research. The limiting factor for aDNA studies is the degree of DNA preservation. Our study on 291 prehistoric cattle remains from Europe, the Near East and North Africa revealed that DNA preservation is mainly influenced by geographic and climatic conditions. Especially in hot climates, the preservation of sample material is generally low. We observed that these specimens are prone to further degradation and contamination during and after excavation. We give a description of the main caveats and a short guideline for adequate sample handling in order to facilitate the cooperation between archaeologists and geneticists and to improve the outcome of future research.

引用

页码：91 / 98

页数：8

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