Differential mRNA expression of the related extracellular matrix glycoproteins SC1 and SPARC in the rat embryonic nervous system and skeletal structure

被引：21

作者：

Mothe, AJ ^{[1
]}

Brown, IR ^{[1
]}

机构：

[1] Univ Toronto, Dept Zool, Toronto, ON M1C 1A4, Canada

来源：

BRAIN RESEARCH | 2001年 / 892卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

anti-adhesive; ECM; in situ hybridization; spinal cord; neural development;

D O I：

10.1016/S0006-8993(00)03141-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

SPARC is a multifunctional extracellular matrix glycoprotein that shares partial sequence homology with SC1. These extracellular matrix molecules are thought to play important roles in modulating cellular interactions. In vitro, SPARC has been shown to exhibit anti-adhesive activity. In the present investigation, in situ hybridization is used to compare the expression patterns of SC1 and SPARC mRNA in the rat embryo. Results show that SC1 and SPARC expression is spatially and temporally regulated. SC1 mRNA is strongly expressed in the embryonic brain and spinal cord, whereas SPARC mRNA is enriched in craniofacial cartilage and skeletal structures. This differential expression pattern in the rat embryo suggests that SC1 plays an important role in the developing nervous system, whereas SPARC participates primarily in events associated with skeletal development. However at embryonic day 17, SC1 and SPARC mRNA show parallel expression patterns in areas of the cerebellum undergoing cell migratory events. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：27 / 41

页数：15

共 41 条

[21] SC1, A SPARC-related glycoprotein, exhibits features of an ECM component in the developing and adult brain [J].