Versatile, fully automated, microfluidic cell culture system

被引：483

作者：

Gomez-Sjoeberg, Rafael

Leyrat, Anne A.

Pirone, Dana M.

Chen, Christopher S.

Quake, Stephen R. ^{[1
]}

机构：

[1] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[2] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

[3] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 22期

关键词：

D O I：

10.1021/ac071311w

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation, osteogenic differentiation, and motility of human primary mesenchymal stem cells.

引用

页码：8557 / 8563

页数：7

共 26 条

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