Poly-γ-glutamate synthetase of Bacillus subtilis

被引：50

作者：

Ashiuchi, M ^{[1
]}

Kamei, T ^{[1
]}

Misono, H ^{[1
]}

机构：

[1] Kochi Univ, Dept Bioresources Sci, Nankoku, Kochi 7838502, Japan

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2003年 / 23卷 / 2-6期

关键词：

poly-gamma-glutamate; synthetase; amide ligase; membranous enzyme; complex; Bacillus subtilis;

D O I：

10.1016/S1381-1177(03)00076-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly-gamma-glutamate (PGA) is a most promising biodegradable polymer. In extracellular mucilage-producing Bacillus subtilis, the pgsBCA genes encode the membrane-associated PGA synthetase complex. It was recently speculated that PGA synthetase consists of both the intact 44 kDa and the in-phase overlapping 33 kDa-ywsC (corresponding to pgsB) gene products. This review covers current research into B. subtilis PGA synthetase and discusses the structural and functional features of the enzyme. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：101 / 106

页数：6

共 32 条

[1] Purification and properties of two isozymes of gamma-glutamyltranspeptidase from Bacillus subtilis TAM-4 [J].

Abe, K ;

Ito, Y ;

Ohmachi, T ;

Asada, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (10) :1621-1625

[2] Purification of Synechocystis sp strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity [J].

Aboulmagd, E ;

Sanio, FBO ;

Steinbüchel, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (05) :2176-2182

[3] CHARACTERIZATION OF STRUCTURAL COMPONENT OF CELL-WALLS OF ALKALOPHILIC STRAIN OF BACILLUS SP C-125 - PREPARATION OF POLY(GAMMA-L-GLUTAMATE) FROM CELL-WALL COMPONENT [J].

AONO, R .

BIOCHEMICAL JOURNAL, 1987, 245 (02) :467-472

[4] A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336:: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells [J].

Ashiuchi, M ;

Soda, K ;

Misono, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 263 (01) :6-12

[5] Isolation of Bacillus subtilis (chungkookjang), a poly-γ-glutamate producer with high genetic competence [J].

Ashiuchi, M ;

Kamei, T ;

Baek, DH ;

Shin, SY ;

Sung, MH ;

Soda, K ;

Yagi, T ;

Misono, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 57 (5-6) :764-769

[6] Biochemistry and molecular genetics of poly-γ-glutamate synthesis [J].

Ashiuchi, M ;

Misono, H .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59 (01) :9-14

[7] Physiological and biochemical characteristics of poly γ-glutamate synthetase complex of Bacillus subtilis [J].

Ashiuchi, M ;

Nawa, C ;

Kamei, T ;

Song, JJ ;

Hong, SP ;

Sung, MH ;

Soda, K ;

Yagi, T ;

Misono, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (20) :5321-5328

[8]

Ashiuchi M., 2002, BIOPOLYMERS, P123

[9] PHYSICOMECHANICAL PROPERTIES OF DEGRADABLE POLYMERS USED IN MEDICAL APPLICATIONS - A COMPARATIVE-STUDY [J].

ENGELBERG, I ;

KOHN, J .

BIOMATERIALS, 1991, 12 (03) :292-304

[10] Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: Identification of a ligase superfamily [J].

Eveland, SS ;

Pompliano, DL ;

Anderson, MS .

BIOCHEMISTRY, 1997, 36 (20) :6223-6229

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