An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration

被引：160

作者：

Wu, Jinglei ^{[1
,2
]}

Ding, Qing ^{[1
,2
]}

Dutta, Ahana ^{[1
,2
]}

Wang, Yezhou ^{[1
,2
]}

Huang, Yi-hui ^{[1
,2
]}

Weng, Hong ^{[1
]}

Tang, Liping ^{[1
,2
]}

Hong, Yi ^{[1
,2
]}

机构：

[1] Univ Texas Arlington, Dept Bioengn, Arlington, TX 76019 USA

[2] Univ Texas SW Med Ctr Dallas, Joint Grad Biomed Engn Program, Dallas, TX 75390 USA

来源：

ACTA BIOMATERIALIA | 2015年 / 16卷

关键词：

Porcine meniscus; Decellularization; Extracellular matrix; Injectable hydrogel; Meniscus regeneration; TISSUE-ENGINEERING APPLICATIONS; PORCINE MEDIAL MENISCUS; RAT BONE-MARROW; IN-VITRO; COLLAGEN FIBRILLOGENESIS; CELL BEHAVIOR; SHEEP MODEL; STEM-CELLS; SCAFFOLD; CARTILAGE;

D O I：

10.1016/j.actbio.2015.01.027

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Tissue-derived extracellular matrix (ECM) biomaterials to regenerate the meniscus have gained increasing attention in treating meniscus injuries and diseases, particularly for aged persons and athletes. However, ECM scaffold has poor cell infiltration and can only be implanted using surgical procedures. To overcome these limitations, we developed an injectable ECM hydrogel material from porcine meniscus via modified decellularization and enzymatic digestion. This meniscus-derived ECM hydrogel exhibited a fibrous morphology with tunable compression and initial modulus. It had a good injectability evidenced by syringe injection into mouse subcutaneous tissue. The hydrogel showed good cellular compatibility by promoting the growth of both bovine chondrocytes and mouse 3T3 fibroblasts encapsulated in the hydrogel for 2 weeks. It also promoted cell infiltration as shown in both in vitro cell culture and in vivo mouse subcutaneous implantation. The in vivo study revealed that the ECM hydrogel possessed good tissue compatibility after 7 days of implantation. The results support the great potential of the newly produced injectable meniscus-derived ECM hydrogel specifically for meniscus repair and regeneration. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：49 / 59

页数：11

共 74 条

[1]

Agrawal V, 2011, TISSUE ENG PT A, V17, P2435, DOI [10.1089/ten.tea.2011.0036, 10.1089/ten.TEA.2011.0036]

[2]

Azhim A, 2013, IEEE ENG MED BIO, P6953, DOI 10.1109/EMBC.2013.6611157

[3]

Biodegradation of high-toughness double network hydrogels as potential materials for artificial cartilage [J].

Azuma, Chinatsu ;

Yasuda, Kazunori ;

Tanabe, Yoshie ;

Taniguro, Hiroko ;

Kanaya, Fuminori ;

Nakayama, Atsushi ;

Chen, Yong Mei ;

Gong, Jian Ping ;

Osada, Yoshihito .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 81A (02) :373-380

[4]

RETRACTED: Engineered whole organs and complex tissues (Retracted article. See vol. 392, pg. 11, 2018) [J].

Badylak, Stephen F. ;

Weiss, Daniel J. ;

Caplan, Arthur ;

Macchiarini, Paolo .

LANCET, 2012, 379 (9819) :943-952

[5]

The effect of nanofiber alignment on the maturation of engineered meniscus constructs [J].

Baker, Brendon M. ;

Mauck, Robert L. .

BIOMATERIALS, 2007, 28 (11) :1967-1977

[6]

Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold [J].

Blakeney, Bryan A. ;

Tambralli, Ajay ;

Anderson, Joel M. ;

Andukuri, Adinarayana ;

Lim, Dong-Jin ;

Dean, Derrick R. ;

Jun, Ho-Wook .

BIOMATERIALS, 2011, 32 (06) :1583-1590

[7]

Brightman AO, 2000, BIOPOLYMERS, V54, P222

[8]

Confined compression of collagen hydrogels [J].

Busby, Grahame A. ;

Grant, M. Helen ;

MacKay, Simon P. ;

Riches, Philip E. .

JOURNAL OF BIOMECHANICS, 2013, 46 (04) :837-840

[9]

Compressive moduli of the human medial meniscus in the axial and radial directions at equilibrium and at a physiological strain rate [J].

Chia, Helena N. ;

Hull, M. L. .

JOURNAL OF ORTHOPAEDIC RESEARCH, 2008, 26 (07) :951-956

[10]

A tissue engineering approach to meniscus regeneration in a sheep model [J].

Chiari, C. ;

Koller, U. ;

Dorotka, R. ;

Eder, C. ;

Plasenzotti, R. ;

Lang, S. ;

Ambrosio, L. ;

Tognana, E. ;

Kon, E. ;

Salter, D. ;

Nehrer, S. .

OSTEOARTHRITIS AND CARTILAGE, 2006, 14 (10) :1056-1065

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