Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser

被引:100
作者
Pan, YL [1 ]
Holler, S
Chang, RK
Hill, SC
Pinnick, RG
Niles, S
Bottiger, JR
机构
[1] Yale Univ, Dept Appl Phys, New Haven, CT 06520 USA
[2] Yale Univ, Ctr Laser Diagnost, New Haven, CT 06520 USA
[3] USA, Res Lab, Adelphi, MD 20783 USA
[4] USA, Edgewood Res Dev & Engn Ctr, Aberdeen Proving Ground, MD 21010 USA
关键词
D O I
10.1364/OL.24.000116
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Reproducible fluorescence spectra of individual 2- to 5-mu m-diameter biological aerosol particles excited with a single shot from a Q-switched laser (266 or 351 nm) have been obtained with highly improved signal-to-noise ratios. Critical to the advance are crossed diode-laser trigger beams, which precisely define the sample volume, and a reflecting objective, which minimizes chromatic aberration and has a large N.A. for collecting fluorescence. Several allergens (red oak, meadow oat pollen, paper mulberry pollen, and puffball spores) have different fluorescence spectra. Bacillus subtilis fluorescence spectrum deteriorates at high 266-nm incident intensity. Dry riboflavin particles illuminated with a 351-nm light exhibit a new 420-nm fluorescence peak that grows nonlinearly with laser pulse energy. (C) 1999 Optical Society of America.
引用
收藏
页码:116 / 118
页数:3
相关论文
共 8 条
  • [1] Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation
    Chen, G
    Nachman, P
    Pinnick, RG
    Hill, SC
    Chang, RK
    [J]. OPTICS LETTERS, 1996, 21 (16) : 1307 - 1309
  • [2] Spectrally resolved absolute fluorescence cross sections for Bacillus spores
    Faris, GW
    Copeland, RA
    Mortelmans, K
    Bronk, BV
    [J]. APPLIED OPTICS, 1997, 36 (04): : 958 - 967
  • [3] Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence
    Hairston, PP
    Ho, J
    Quant, FR
    [J]. JOURNAL OF AEROSOL SCIENCE, 1997, 28 (03) : 471 - 482
  • [4] Two-dimensional angular optical scattering for the characterization of airborne microparticles
    Holler, S
    Pan, YL
    Chang, RK
    Bottiger, JR
    Hill, SC
    Hillis, DB
    [J]. OPTICS LETTERS, 1998, 23 (18) : 1489 - 1491
  • [5] FLUORESCENCE PARTICLE COUNTER FOR DETECTING AIRBORNE BACTERIA AND OTHER BIOLOGICAL PARTICLES
    PINNICK, RG
    HILL, SC
    NACHMAN, P
    PENDLETON, JD
    FERNANDEZ, GL
    MAYO, MW
    BRUNO, JG
    [J]. AEROSOL SCIENCE AND TECHNOLOGY, 1995, 23 (04) : 653 - 664
  • [6] Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles
    Pinnick, RG
    Hill, SC
    Nachman, P
    Videen, G
    Chen, G
    Chang, RK
    [J]. AEROSOL SCIENCE AND TECHNOLOGY, 1998, 28 (02) : 95 - 104
  • [7] Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution
    Seaver, M
    Roselle, DC
    Pinto, JF
    Eversole, JD
    [J]. APPLIED OPTICS, 1998, 37 (22): : 5344 - 5347
  • [8] SEAVER M, IN PRESS AEROSOL SCI