A tunable FRET circuit for engineering fluorescent biosensors

被引：46

作者：

Allen, Michael D. ^{[1
,2
,3
]}

Zhang, Jin ^{[1
,2
,3
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 03期

关键词：

fluorescence; FRET (fluorescence resonant energy transfer); kinases; microscopy;

D O I：

10.1002/anie.200703493

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Donor, acceptor, and tuner: FRET-based ratiometric live-cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET-circuit-based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors. © 2008 Wiley-VCH Verlag GmbH &. Co. KGaA.

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收藏

页码：500 / 502

页数：3

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