Analysis of cocaine, benzoylecgonine, codeine, and morphine in hair by supercritical fluid extraction with carbon dioxide modified with methanol

被引:40
作者
Brewer, WE
Galipo, RC
Sellers, KW
Morgan, SL [1 ]
机构
[1] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
[2] S Carolina Law Enforcement Div, Dept Toxicol, Columbia, SC 29210 USA
关键词
D O I
10.1021/ac000871r
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Supercritical fluid extraction (SFE) using CO2 modified with methanol (10%) was employed for the extraction of cocaine from human hair. Extraction conditions were developed by using designed experiments to characterize the effects of pressure, temperature, and percent methanol on the recovery of cocaine from hair. When compared to the conventional acid hydrolysis method for hair analysis, SFE was faster and gave higher recoveries. Amounts of cocaine, benzoylecgonine (cocaine metabolite), codeine, and morphine in a hair standard reference material were determined by SEE-gas chromatography/ mass spectrometry (GC/MS) and found to be in agreement with reported levels. Analyses of hair from forensic case studies are also reported.
引用
收藏
页码:2371 / 2376
页数:6
相关论文
共 22 条
[11]  
KINTZ P, 1996, DRUG TESTING HAIR
[12]   ROLE OF MODIFIERS FOR ANALYTICAL-SCALE SUPERCRITICAL-FLUID EXTRACTION OF ENVIRONMENTAL-SAMPLES [J].
LANGENFELD, JJ ;
HAWTHORNE, SB ;
MILLER, DJ ;
PAWLISZYN, J .
ANALYTICAL CHEMISTRY, 1994, 66 (06) :909-916
[13]   SOLID-PHASE EXTRACTION WITH SUPERCRITICAL FLUID ELUTION AS A SAMPLE PREPARATION TECHNIQUE FOR THE ULTRATRACE ANALYSIS OF FLAVONE IN BLOOD-PLASMA [J].
LIU, HJ ;
WEHMEYER, KR .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 577 (01) :61-67
[14]   COMBINED SUPERCRITICAL FLUID EXTRACTION SOLID-PHASE EXTRACTION WITH OCTADECYLSILANE CARTRIDGES AS A SAMPLE PREPARATION TECHNIQUE FOR THE ULTRATRACE ANALYSIS OF A DRUG METABOLITE IN PLASMA [J].
LIU, HJ ;
COOPER, LM ;
RAYNIE, DE ;
PINKSTON, JD ;
WEHMEYER, KR .
ANALYTICAL CHEMISTRY, 1992, 64 (07) :802-806
[15]   Matrix and modifier effects in the supercritical fluid extraction of cocaine and benzoylecgonine from human hair [J].
Morrison, JF ;
Chesler, SN ;
Yoo, WJ ;
Selavka, CM .
ANALYTICAL CHEMISTRY, 1998, 70 (01) :163-172
[16]  
PARKS OW, 1995, J CHROMATOGR SCI, V33, P654
[17]   ISOLATION OF SULFONAMIDES FROM FORTIFIED CHICKEN TISSUES WITH SUPERCRITICAL CO2 AND IN-LINE ADSORPTION [J].
PARKS, OW ;
MAXWELL, RJ .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1994, 32 (07) :290-293
[18]   SUPERCRITICAL-FLUID EXTRACTION OF S-TRIAZINES AND PHENYLUREA HERBICIDES FROM SEDIMENT [J].
ROBERTSON, AM ;
LESTER, JN .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1994, 28 (02) :346-351
[19]   Interlaboratory studies on the analysis of hair for drugs of abuse: Results from the fourth exercise [J].
Sniegoski, LT ;
Welch, MJ .
JOURNAL OF ANALYTICAL TOXICOLOGY, 1996, 20 (04) :242-247
[20]   SIMULTANEOUS ASSAY OF COCAINE, HEROIN AND METABOLITES IN HAIR, PLASMA, SALIVA AND URINE BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].
WANG, WL ;
DARWIN, WD ;
CONE, EJ .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 660 (02) :279-290