In vitro biocompatibility of titanium oxide for prosthetic devices nanostructured by low pressure metal-organic chemical vapor deposition

被引:37
作者
Giavaresi, G
Giardino, R
Ambrosio, L
Battiston, G
Gerbasi, R
Fini, M
Rimondini, L
Torricelli, R
机构
[1] IOR, Ist Ric Codivilla Putti, Serv Chirurg Sperimentale, Dept Expt Surg, I-40136 Bologna, Italy
[2] Univ Bologna, Fac Med, Chair Surg Pathophysiol, Bologna, Italy
[3] Univ Naples Federico II, CNR, Inst Composite & Biomed Mat, Naples, Italy
[4] Univ Padua, CNR, Inst Inorgan & Surface Chem, Padua, Italy
关键词
titanium oxide; orthopedics; implants; osteoblast-like cells; biocompatibility;
D O I
10.1177/039139880302600811
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Metal-Organic Chemical Vapor Deposition (MOCVD) has recently been proposed to coat orthopedic and dental prostheses with metal nanostructured oxide films through the decomposition of oxygenated compounds (single-source precursors) or the reaction of oxygen-free metal compounds with oxygenating agents. The present study was carried out to assess the in vitro biocompatibility in terms of cell proliferation and activation, of commercially pure Ti (control material: TI/MA) coated with nanostructured TiO2 film by MOCVD (Ti/MOCVD) using osteoblast-like cell cultures (MG-63). Evaluations were performed at 3, 7 and 14 days. Cell proliferation showed a similar trend for Ti/MA and TiIMOCVD compared to polystyrene; cell number increased with time from seeding to day 7 (p < 0.005), and then decreased progressively until day 14 (ranging from -14% to -47%). The ALP level and OC production showed no significant differences between TiIMOCVD and Ti/MA at each experimental time. Significantly higher ALP levels were found in Ti/MA at 3 days and in Ti/MOCVD at 7 and 14 days when compared to the polystyrene group. OC production decreased over time and the highest values were observed at 3 days, when it was significantly higher in the Ti/MA than in the polystyrene group (50%, p < 0.05). CICP synthesis was positively affected by the presence of TiIMOCVD and was higher in TiIMOCVD than in the polystyrene group. No significant differences were found between TiIMOCVD and Ti/MA in terms of IL-6 and TGF-beta1 synthesis at any experimental time. In conclusion, the current findings demonstrate that the nanostructured TiO2 coating positively affects the osteoblast-like cell behavior in terms of cell proliferation and activity, thus confirming its high level of in vitro biocompatibility in accordance with expectations.
引用
收藏
页码:774 / 780
页数:7
相关论文
共 49 条
  • [41] STANFORD C M, 1991, Critical Reviews in Oral Biology and Medicine, V2, P83
  • [42] BIOCOMPATIBILITY OF CHEMICAL-VAPOR-DEPOSITED DIAMOND
    TANG, L
    TSAI, C
    GERBERICH, WW
    KRUCKEBERG, L
    KANIA, DR
    [J]. BIOMATERIALS, 1995, 16 (06) : 483 - 488
  • [43] TISEL CL, 1994, J BONE JOINT SURG AM, V76, P139
  • [44] Toni A, 2001, HIP INT, V11, P1
  • [45] Tripi TR, 2002, J ENDODONT, V28, P497
  • [46] Wen XJ, 1996, BIO-MED MATER ENG, V6, P173
  • [47] APPLICATION OF PVD TIN COATING TO CO-CR-MO BASED SURGICAL IMPLANTS
    WISBEY, A
    GREGSON, PJ
    TUKE, M
    [J]. BIOMATERIALS, 1987, 8 (06) : 477 - 480
  • [48] ORGANOMETALLIC PRECURSORS IN THE GROWTH OF EPITAXIAL THIN-FILMS OF GROUPS III-V SEMICONDUCTORS BY METAL ORGANIC-CHEMICAL VAPOR-DEPOSITION
    ZANELLA, P
    ROSSETTO, G
    BRIANESE, N
    OSSOLA, F
    PORCHIA, M
    WILLIAMS, JO
    [J]. CHEMISTRY OF MATERIALS, 1991, 3 (02) : 225 - 242
  • [49] Dissolution/reprecipitation of calcium phosphate thin films produced by ion beam sputter deposition technique
    Zeng, H
    Chittur, KK
    Lacefield, WR
    [J]. BIOMATERIALS, 1999, 20 (05) : 443 - 451