Metagenomic comparison of direct and indirect soil DNA extraction approaches

被引：76

作者：

Delmont, Tom O.

Robe, Patrick ^{[2
]}

Clark, Ian ^{[3
]}

Simonet, Pascal

Vogel, Timothy M. ^{[1
]}

机构：

[1] Univ Lyon, Lab AMPERE, Ecole Cent Lyon, F-69134 Ecully, France

[2] LibraGen, F-31400 Toulouse, France

[3] Rothamsted Res, Harpenden AL5 2JQ, Herts, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 86卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Soil metagenomics; DNA extraction; Nycodenz; MOLECULAR-WEIGHT DNA; ENVIRONMENTAL DNA; PURIFICATION; QUANTIFICATION; RECOVERY;

D O I：

10.1016/j.mimet.2011.06.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same. (C) 2011 Elsevier B.V. All rights reserved.

引用

收藏

页码：397 / 400

页数：4

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