Glucocorticoid receptor mutants: man-made tools for functional research

被引：37

作者：

Beck, Ilse M. ^{[1
]}

De Bosscher, Karolien ^{[1
]}

Haegeman, Guy ^{[1
]}

机构：

[1] Univ Ghent, Dept Physiol, LEGEST, B-9000 Ghent, Belgium

来源：

TRENDS IN ENDOCRINOLOGY AND METABOLISM | 2011年 / 22卷 / 08期

关键词：

NF-KAPPA-B; LIGAND-BINDING DOMAIN; STEROID-HORMONE RECEPTORS; GASTRIC-ACID-SECRETION; DNA-BINDING; TRANSCRIPTIONAL ACTIVATION; NUCLEAR EXPORT; GENE-EXPRESSION; HSP90; BINDING; HINGE REGION;

D O I：

10.1016/j.tem.2011.03.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The glucocorticoid receptor (GR) is a ligand-dependent transcription factor that can bind to glucocorticoids (GCs). Upon ligand binding, GR sheds its cytoplasmic chaperoning complex and translocates to the nucleus, where it can act as a ligand-dependent transcription factor, transactivating or transrepressing specific gene promoters. Often, GR interacts with specific cofactors to implement a variety of gene promoter effects. GR activity and function is further modulated by post-translational modifications. To assess the diverse aspects of GR mechanisms of activation and gene regulation, researchers continue to use a range of artificial GR mutants. In this review we analyze the characteristics of GR mutants with the aim of assisting the design and interpretation of GR mutant-based experiments.

引用

页码：295 / 310

页数：16

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