Helicobacter pylori genotypes may determine gastric histopathology

被引:144
作者
Nogueira, C
Figueiredo, C
Carneiro, F
Gomes, AT
Barreira, R
Figueira, P
Salgado, C
Belo, L
Peixoto, A
Bravo, JC
Bravo, LE
Realpe, JL
Plaisier, AP
Quint, WGV
Ruiz, B
Correa, P
van Doorn, LJ
机构
[1] Louisiana State Univ, Dept Pathol, New Orleans, LA USA
[2] Erasmus Univ, Dept Publ Hlth, Fac Med, Rotterdam, Netherlands
[3] Univ Valle, Cali, Colombia
[4] ENVC, Viana Do Catelo, Portugal
[5] Hosp S Joao, Porto, Portugal
[6] Delft Diagnost Lab, NL-2625 AD Delft, Netherlands
[7] Univ Porto, Fac Med, P-4100 Porto, Portugal
[8] Univ Porto, Inst Mol Pathol & Immunol, P-4100 Porto, Portugal
关键词
D O I
10.1016/S0002-9440(10)64006-0
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The outcome of Helicobacter pylori infection has been associated with specific virulence-associated bacterial genotypes, The present study aimed to investigate the gastric histopathology in Portuguese and Colombian patients infected with H, pylori and to assess its relationship with bacterial virulence-associated vacA, cagA, and iceA genotypes, A total of 370 patients from Protugal (n = 192) and Colombia(n = 178) were studied. Corpus and antrum biopsy specimens were collected from each individual. Histopathological features were recorded and graded according to the updated Sydney system. H, pylori vacA, cagA, and iceA genes were directly genotyped in the gastric biopsy specimens by polymerase chain reaction and reverse hybridization. Despite the significant differences between the Portuguese and Colombian patient groups, highly similar results were observed with respect to the relation between H. pylori genotypes and histopathology, n, pylori vacA s1, vacA ml,cagA + genotypes were significantly associated with a higher H. pylori density, higher degrees of lymphocytic and neutrophilic infiltrates, atrophy, the type of intestinal metaplasia, and presence of epithelial damage. The iceA1 genotype was only associated with epithelial damage in Portuguese patients. These findings show that distinct H, pylori genotypes are strongly associated with histopathological findings in the stomach, confirming their relevance for the development of H. pylori-associated gastric pathology.
引用
收藏
页码:647 / 654
页数:8
相关论文
共 49 条
[11]   cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].
Censini, S ;
Lange, C ;
Xiang, ZY ;
Crabtree, JE ;
Ghiara, P ;
Borodovsky, M ;
Rappuoli, R ;
Covacci, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653
[12]  
CORREA P, 1988, CANCER RES, V48, P3554
[13]  
CORREA P, 1992, CANCER RES, V52, P6735
[14]  
CORREA P, 1995, AM J SURG PATHOL, V19, P37
[15]   SEROLOGIC DETECTION OF INFECTION WITH CAGA(+) HELICOBACTER-PYLORI STRAINS [J].
COVER, TL ;
GLUPCZYNSKI, Y ;
LAGE, AP ;
BURETTE, A ;
TUMMURU, MKR ;
PEREZPEREZ, GI ;
BLASER, MJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (06) :1496-1500
[16]   The vacuolating cytotoxin of Helicobacter pylori [J].
Cover, TL .
MOLECULAR MICROBIOLOGY, 1996, 20 (02) :241-246
[17]   Classification and grading of gastritis - The updated Sydney System [J].
Dixon, MF ;
Genta, RM ;
Yardley, JH ;
Correa, P ;
Batts, KP ;
Dahms, BB ;
Filipe, MI ;
Haggitt, RC ;
Haot, J ;
Hui, PK ;
Lechago, J ;
Lewin, K ;
Offerhaus, JA ;
Price, AB ;
Riddell, RH ;
Sipponen, P ;
Solcia, E ;
Watanabe, H .
AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1996, 20 (10) :1161-1181
[18]   Helicobacter pylori [J].
Dunn, BE ;
Cohen, H ;
Blaser, MJ .
CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (04) :720-+
[19]   Helicobacter pylori cagA status and s and m alleles of vacA in isolates from individuals with a variety of H-pylori associated gastric diseases [J].
Evans, DG ;
Queiroz, DMM ;
Mendes, EN ;
Evans, DJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) :3435-3437
[20]   Genetic organization and heterogeneity of the iceA locus of Helicobacter pylori [J].
Figueiredo, C ;
Quint, WGV ;
Sanna, R ;
Sablon, E ;
Donahue, JP ;
Xu, Q ;
Miller, GG ;
Peek, RM ;
Blaser, MJ ;
van Doorn, LJ .
GENE, 2000, 246 (1-2) :59-68